BM2274F CD326 / EPCAM / TACSTD1 antibody

See related secondary antibodies

Search for all "CD326 / EPCAM / TACSTD1"

0.25 ml / €410.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD326 / EPCAM / TACSTD1 HEA125

BM2274F

Product Description for CD326 / EPCAM / TACSTD1

Mouse anti Human CD326 / EPCAM / TACSTD1 HEA125.
Presentation: FITC
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for CD326 / EPCAM / TACSTD1

Product Category Primary Antibodies
Target Category
Quantity 0.25 ml
Synonyms Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
Presentation FITC
Reactivity Hu
Applications C, ICC/IF, P
Clonality Monoclonal
Clone HEA125
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P16422
Immunogen:
HT-29 colon carcinoma cell line
GeneID:
4072
Isotype control SM10F (for use in human samples)
Application Immunofluorescence Microscopy.
Cell Sorting.
Cytological Material.
Immunohistochemistry on Frozen Sections and Paraffin-Embedded Sections.

With Paraffin embedded sections, protease pretreatment is required prior to antibody application.
Working Dilution: Dilute at least 1/10 with PBS, pH 7.4 for Immunohistochemical application.
Incubation Time: 1 h at RT.
Background Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.
General Readings
  1. Edwards PA. Heterogeneous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies. Br J Cancer. 1985 Feb;51(2):149-60. PubMed PMID: 2578284. (Free PMC Article available)
  2. Moldenhauer G, Momburg F, Möller P, Schwartz R, Hämmerling GJ. Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br J Cancer. 1987 Dec;56(6):714-21. PubMed PMID: 2449234. (Free PMC Article available)
  3. Momburg F, Moldenhauer G, Hämmerling GJ, Möller P. Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues. Cancer Res. 1987 Jun 1;47(11):2883-91. PubMed PMID: 3552208.
  4. Simon B, Podolsky DK, Moldenhauer G, Isselbacher KJ, Gattoni-Celli S, Brand SJ. Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2755-9. PubMed PMID: 2108441. (Free PMC Article available)
  5. Kemmner W, Moldenhauer G, Schlag P, Brossmer R. Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads. J Immunol Methods. 1992 Mar 4;147(2):197-200. PubMed PMID: 1548402.
  6. Winter MJ, Nagtegaal ID, van Krieken JH, Litvinov SV. The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am J Pathol. 2003 Dec;163(6):2139-48. PubMed PMID: 14633587. (Free PMC Article available, 2 images available)
  7. Joplin R, Strain AJ, Neuberger JM. Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: isolation, characterization, and short-term culture. J Pathol. 1990 Nov;162(3):255-60. PubMed PMID: 2266463.
  8. Metzgeroth G, Mantz C, Kuhn C, Schultheis B, Hehlmann R, Hastka J. Reliable identification of small cell lung cancer in cytological specimens by immunocytology. Onkologie. 2007 Jun;30(6):311-5. Epub 2007 May 29. PubMed PMID: 17551254.
  9. Kommoss F, Oliva E, Young RH, Bittinger F, Kirkpatrick CJ, Schmidt D: Expression of Müllerian inhibiting substance, CD99 and HEA125 in ovarian tumors. Geburtsh Frauenheik 61, 274-279 (2001).
  10. Racila E, Euhus D, Weiss AJ, Rao C, McConnell J, Terstappen LW, et al. Detection and characterization of carcinoma cells in the blood. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4589-94. PubMed PMID: 9539782. (Free PMC Article available, 4 images available)
  11. Balzar M, Winter MJ, de Boer CJ, Litvinov SV. The biology of the 17-1A antigen (Ep-CAM). J Mol Med (Berl). 1999 Oct;77(10):699-712. PubMed PMID: 10606205.
  12. Hastka J, Pfiester P. [Cell marker for the differential diagnosis of malignant mesotheliomas]. Pathologe. 1988 Jul;9(4):245-7. PubMed PMID: 2463617.
  13. Momburg F, Moldenhauer G, Hämmerling GJ, Möller P, Otto HF: Charakterisierung eines epithelspezifischen Zellmembranantigens mit Hilfe des monoklonalen Antikörpers HEA125. Verh Dtsch Ges Path 69, 627 (1985)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS, pH 7.4
Preservatives:
0.09% Sodium Azide
State:
Liquid purified Ig fraction
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen or EPG34). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins).
A subset of squamous cell carcinoma is negative.
Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116).
Polypeptide Reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM).
Gene ID 4072
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for CD326 / EPCAM / TACSTD1 (8 products)

Catalog No. Species Pres. Purity   Source  

CD326 / EPCAM / TACSTD1

CD326 / EPCAM / TACSTD1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD326 / EPCAM / TACSTD1

CD326 / EPCAM / TACSTD1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €399.00
  OriGene Technologies, Inc.

CD326 / EPCAM / TACSTD1 (24-265, His-tag)

CD326 / EPCAM / TACSTD1 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

CD326 / EPCAM / TACSTD1 (24-265, His-tag)

CD326 / EPCAM / TACSTD1 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

CD326 / EPCAM / TACSTD1

CD326 / EPCAM / TACSTD1 Human
10 µg / €540.00
  Abnova Taiwan Corp.

CD326 / EPCAM / TACSTD1

CD326 / EPCAM / TACSTD1 Human Human HEK293T cells
2 µg / €590.00
  Abnova Taiwan Corp.

CD326 / EPCAM / TACSTD1

CD326 / EPCAM / TACSTD1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CD326 / EPCAM / TACSTD1

CD326 / EPCAM / TACSTD1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Positive controls for CD326 / EPCAM / TACSTD1 (4 products)

Catalog No. Species Pres. Purity   Source  

EpCAM Lysate

Western Blot: EpCAM Lysate [NBL1-16674] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for EPCAM
0.1 mg / €290.00
  Novus Biologicals Inc.

EPCAM overexpression lysate

EPCAM overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.

TACSTD1 293T Cell Transient Overexpression Lysate(Denatured)

TACSTD1 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €270.00
  Abnova Taiwan Corp.

TACSTD1 293T Cell Transient Overexpression Lysate(Denatured)

TACSTD1 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €270.00
  Abnova Taiwan Corp.
  • LinkedIn