AM32448PU-N CD34 antibody

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0.1 mg / €330.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Sheep CD34 EQ-8D11-C1


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  • AM32448PU-N

Product Description for CD34

Mouse anti Sheep CD34 EQ-8D11-C1.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for CD34

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
Presentation Purified
Reactivity Sh
Applications E, F, ICC/IF
Clonality Monoclonal
Clone EQ-8D11-C1
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Genetic immunisation with cDNA encoding the extracellular domain of Ovine CD34
Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells
Application Flow Cytometry: 1.2 μg/106 cells. 
Immunofluorescence: 1 μg/106 cells.  
Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
Background CD34 is a type I membrane protein with an apparent MW of approximately 115 kDa. It is a putative adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells (2). It is thought to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.
Concentration 1.0 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Harbuzariu A, Kim J, Meyer EM, Donnenberg AD, Tillman BW. CD34 affinity pheresis attenuates a surge among circulating progenitor cells following vascular injury. J Vasc Surg. 2014 Jun;59(6):1686-94. doi: 10.1016/j.jvs.2013.05.043. Epub 2013 Jul 19. PubMed PMID: 23876509.
  2. Porada CD, Harrison-Findik DD, Sanada C, Valiente V, Thain D, Simmons PJ, et al. Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells. Exp Hematol. 2008 Dec;36(12):1739-49. doi: 10.1016/j.exphem.2008.09.003. PubMed PMID: 19007686. (Free PMC Article available, 7 images available)
General Readings
  1. Shaw, SWS et al 2011: Sheep amniotic fluid derived CD34+ stem cells could engraft and restore the hematopoietic system of NSG mice. ISSCR 9th annual meeting, Poster board #2416
  2. Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH. Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol. 1984 Jul;133(1):157-65. PubMed PMID: 6586833.

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.2
Liquid purified Ig fraction
This antibody recognizes Ovine CD34. Other species not tested.
Cellular Localization: Membrane.

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