SM1095 CD34 Class II antibody

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Mouse anti Human, Monkey CD34 Class II QBEND10


Product Description for CD34 Class II

Mouse anti Human, Monkey CD34 Class II QBEND10.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry, Paraffin Sections.

Properties for CD34 Class II

Product Category Primary Antibodies
Quantity 1 ml
Synonyms CD34, Hematopoietic progenitor cell antigen CD34
Presentation Supernatant
Reactivity Hu, Mky
Applications C, F, IP, P
Clonality Monoclonal
Clone QBEND10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Human endothelial vesicles
Application Flow Cytometry: use 5 µl of neat antibody to label 1x106 cells. Immunoprecipitation. Immunohistochemistry on frozen and paraffin embedded sections: 1:10. This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended positive control tissue: Tonsil, bone marrow Westernblot. Other applications not tested. Optimal dilutions should be determined by the user.
General Readings
  1. Fina L, Molgaard HV, Robertson D, Bradley NJ, Monaghan P, Delia D, et al. Expression of the CD34 gene in vascular endothelial cells. Blood. 1990 Jun 15;75(12):2417-26. PubMed PMID: 1693532.
  2. Sopper, S. et al. (1997). Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of Rhesus mon
Storage Store the antibody at 4-8°C or at -20°C for longer. Avoid repeated freezing and thawing. Shelf live: one year from despatch.
Recognises the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein of Mr 110kD expressed on haemopoietic cells and on the established myeloid leukaemic cell line KG1a. QBEND-10 recognises the Class II epitope on CD34. Clone QBEND-10 has been reported as being suitable for use in Western blotting. This antibody shows no cross reactivity with sheep, cow, dog or rat. Clone QBEND-10 is reported to cross react with Rhesus monkey (2).

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