SM1095P CD34 Class II antibody

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Mouse anti Human, Monkey CD34 Class II QBEND10


Product Description for CD34 Class II

Mouse anti Human, Monkey CD34 Class II QBEND10.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for CD34 Class II

Product Category Primary Antibodies
Quantity 1 ml
Presentation Purified
Reactivity Hu, Mky
Applications C, F, IP, P, WB
Clonality Monoclonal
Clone QBEND10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Human endothelial vesicles
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow Cytometry: use 10 µl of neat antibody to label 1x106 cells in 100 µl. Immunohistochemistry on frozen and paraffin embedded sections (1:50 - 1:100). This product does not require antigen retrieval using heat treatment or protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Recommended positive control: Tonsil, bone marrow. Immunoprecipitation and Western Blot. Other applications not tested. Optimal dilutions should be determined by the user.
Concentration 0.1 mg/ml
General Readings
  1. Fina L, Molgaard HV, Robertson D, Bradley NJ, Monaghan P, Delia D, et al. Expression of the CD34 gene in vascular endothelial cells. Blood. 1990 Jun 15;75(12):2417-26. PubMed PMID: 1693532.
  2. Sopper, S. et al. (1997). Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of Thesus mon
Storage Store the antibody at 4-8°C or at -20°C for longer. This product should be stored undiluted. Avoid repeated freezing and thawing. Shelf live: one year from despatch.
This antibody recognises the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane.
QBEND-10 immunoprecipitates a glycoprotein of Mr 110kD expressed on haemopoietic cells and on the established myeloid leukemic cell line KG1a. QBEND-10 recognises the Class II epitope on CD34.
This clone shows no cross reactivity with sheep, cow, dog or rat. Clone is reported to cross react with Rhesus monkey (2). Antibody reactivity and working conditions may vary between species.

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