NB600-1071C CD34 (MEC 14.7) - Hematopoietic Stem Cell Marker antibody

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Rat anti Mouse CD34 (MEC 14.7) - Hematopoietic Stem Cell Marker MEC 14.7


Product Description for CD34 (MEC 14.7) - Hematopoietic Stem Cell Marker

Rat anti Mouse CD34 (MEC 14.7) - Hematopoietic Stem Cell Marker MEC 14.7.
Presentation: DyLight649
Product is tested for Frozen Sections, Enzyme Immunoassay, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for CD34 (MEC 14.7) - Hematopoietic Stem Cell Marker

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms anti-Cluster designation 34 antibody, anti-HPCA1 antibody, anti-Hematopoietic progenitor cell antigen CD34 an
Presentation DyLight649
Reactivity Ms
Applications C, E, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone MEC 14.7
Host Rat
Isotype IgG2a
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Research Areas: Immunology
Application flow cytometry / FACS analysis, Immunocytochemistry, immunofluorescence, immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot, This antibody can be used in Immunoprecipitation, Immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections, Flow Cytometry, and Western Blot. Antigen retrieval is required for IHC-P., flow cytometry / FACS analysis 1:50 , immunohistochemistry 1:50 , immunofluorescence , immunoprecipitation , Western Blot 1:50, Immunocytochemistry , Immunohistochemistry-Paraffin , Immunohistochemistry-Frozen
Background The antibody is a useful reagent for identification and characterization of capillary endothelial cells. Furthermore the antibody can be used for isolation and characterization of hematopoietic progenitor cells. In summary the antibody can be used for ELISA, immunofluorescence, immunohistochemistry on frozen and paraffin sections, Western blot, immunoprecipitation, flow cytometry and cell separation. Western blot: The reported molecular mass of CD34 is ranging from 100 to 120 kDa, apart from the HEV form (Sgp90) which is about 90 kDa. MEC14.7 can recognize a band of about 100 kDa from the H5V line but a band of 75 to 85 kDa from normal tissue. Since different patterns of glycosylation of the molecule are seen with different cell types, this might explain the wide range of CD34 molecular mass (from 75-85 kDa to 120 kD).
Concentration 0.5 mg/ml
Storage Store at 4C. Do not freeze.
DyLight 649
IgG purified
Buffer System:
No Preservative
IgG purified
The reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries.

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