AM26029FC-N CD4 antibody

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0.1 mg / €180.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rat anti Mouse CD4 GK1.5

AM26029FC-N

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  • AM26029FC-N
  • AM26029FC-N

Product Description for CD4

Rat anti Mouse CD4 GK1.5.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation FITC
Reactivity Ms
Applications F
Clonality Monoclonal
Clone GK1.5
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P06332
Immunogen:
Mouse CTL clone V4 cells
GeneID:
12504
Application Flow cytometry: 3 µg/ml.
Background CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Concentration 0.5 mg/ml
General Readings
  1. Dialynas DP, Wilde DB, Marrack P, Pierres A, Wall KA, Havran W, et al. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 1983;74:29-56. PubMed PMID: 6195085.
  2. Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51. PubMed PMID: 6415170.
  3. Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83. PubMed PMID: 6195255.
  4. Wu L, Antica M, Johnson GR, Scollay R, Shortman K. Developmental potential of the earliest precursor cells from the adult mouse thymus. J Exp Med. 1991 Dec 1;174(6):1617-27. PubMed PMID: 1683894. (Free PMC Article available)
  5. Godfrey DI, Kennedy J, Gately MK, Hakimi J, Hubbard BR, Zlotnik A. IL-12 influences intrathymic T cell development. J Immunol. 1994 Mar 15;152(6):2729-35. PubMed PMID: 7511624.
  6. Gavett SH, Chen X, Finkelman F, Wills-Karp M. Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia. Am J Respir Cell Mol Biol. 1994 Jun;10(6):587-93. PubMed PMID: 8003337.
  7. Zheng B, Han S, Kelsoe G. T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1. J Exp Med. 1996 Sep 1;184(3):1083-91. PubMed PMID: 9064325. (Free PMC Article available)
  8. Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross ML, Suri A, et al. Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes. Nat Immunol. 2007 Apr;8(4):388-97. Epub 2007 Feb 25. PubMed PMID: 17322886.
  9. Hu M, Watson D, Zhang GY, Graf N, Wang YM, Sartor M, et al. Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells. J Immunol. 2008 May 15;180(10):6593-603. PubMed PMID: 18453578.
  10. Yi H, Zhen Y, Zeng C, Zhang L, Zhao Y. Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice. Transplantation. 2008 Apr 27;85(8):1167-74. doi: 10.1097/TP.0b013e31816a1242. PubMed PMID: 18431238.
Storage Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
FITC
Buffer System:
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
State:
Liquid purified Ig fraction
Species Reactivity
Species reactivity (tested):
Mouse
Specificity
Specificity:
This antibody reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
Gene ID 12504

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