AM26060BT-N CD4 antibody

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0.2 mg / €330.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Rat CD4 Rib5/2


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  • AM26060BT-N

Product Description for CD4

Mouse anti Rat CD4 Rib5/2.
Presentation: Biotin
Product is tested for Frozen Sections, Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 0.2 mg
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation Biotin
Reactivity Rt
Applications C, F
Clonality Monoclonal
Clone Rib5/2
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Application Immunohistochemistry on frozen sections: 0.5 μg/ml (1:800).
Does not react on routinely processed paraffin sections.
Has been described to work in FACS.
Suggested positive control: Rat spleen.
General Readings
  1. LEHMANN, M. et al.: Rejektionstherapie mit Anti-CD4 monoklonalen Antikoerpern nach allogener Transplantation bei der Ratte. Fruehjahrstagung fuer Immunologie, Berlin (1991).
  2. KATZKE, G. et al.: Der Effekt einer temporaeren Anti-CD4 Therapie vor und nach allogener Inseltransplantation in Streptozotocin- diabetischen oder spontan-diabetischen Ratten. Fruehjahrstagung fur Immunologie, Berlin (1991).
  3. Lehmann M, Sternkopf F, Metz F, Brock J, Döcke WD, Plantikow A, et al. Induction of long-term survival of rat skin allografts by a novel, highly efficient anti-CD4 monoclonal antibody. Transplantation. 1992 Dec;54(6):959-62. PubMed PMID: 1281565.
  4. Lehmann M, Kuttler B, Siegling A, Gordalla A, Riedel H, Lacha J, et al. Characterization of the anti-CD4-induced permanent acceptance of rat renal allografts. Transplant Proc. 1993 Oct;25(5):2859-60. PubMed PMID: 8212264.
  5. Siegling A, Lehmann M, Riedel H, Platzer C, Brock J, Emmrich F, et al. A nondepleting anti-rat CD4 monoclonal antibody that suppresses T helper 1-like but not T helper 2-like intragraft lymphokine secretion induces long-term survival of renal allografts. Transplantation. 1994 Feb;57(3):464-7. PubMed PMID: 8108886.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
Stock solution contains 0.4 mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5 mg/ml BSA as a stabilizer and 0.01% Kathon as a preservative.
Lyophilized purified Ig fraction
Restore by adding 0.5 ml distilled water.
Species Reactivity
Species reactivity (tested):
The antibody detects a 53kDa glycoprotein located on the cell surface. It is used for the identifcation of CD4 bearing cells, mainly T-helper / inducer lymphocytes. It can also be applied for in vivo blocking of CD4 mediated immunological reactions. 
Antigen Distribution
Isolated Cells: T-lymphocytes and thymocytes, monocytes, macrophages.
Tissue Sections: Rib 5/2 stains T-cell areas in the spleen and lymph nodes. In the thymus Rib 5/2 detects cortical and medullary thymocytes. It recognises tissue macrophages and Th-cells according to the CD4 distribution.
Gene ID 24932

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