AM26438AF-N CD4 antibody

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0.1 mg / €390.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD4 4H5

Product Description for CD4

Mouse anti Human CD4 4H5.
Presentation: Azide Free
Product is tested for Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation Azide Free
Reactivity Hu
Applications F
Clonality Monoclonal
Clone 4H5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01730
Immunogen:
PHA-activated human CD4+ T-cells
GeneID:
920
Add. information This product was originally produced by MBL International.
Application Flow cytometry: 5 μg/ml (final concentration). For details see protocol below.
Background

The T cell receptor for antigen (TCR) is a membrane-bound member of the immunoglobulin superfamily (IgSF) that recognizes a complex of antigen bound to self major histocompatibility complex (MHC) molecules expressed on the cell-surface of antigen-presenting cells (APC). Both cytotoxic and helper T cells typically respond to antigen which is associated with different types of MHC molecules, class I and II, respectively. CD4 is a 55-60 kDa cell-surface glycoprotein, which participates in the molecular complexes involved both in T cell development and its antigen recognizing activity, by binding to the nonpolymorphic region of class II MHC. This interaction results in increased intercellular adhesion and enhanced stimulation of T cells by signal transduction which is mediated by intracellular molecules. CD4 is also considered as a stage marker of T cell development in thymus, for it is expressed on the cell surface in a stage specific manner, during T cell development.

Concentration 1.0 mg/ml
Protocols

Flow cytometric analysis for whole blood cells
We usually use Falcon tubes or equivalents as reaction tubes for all steps described below.
1) Add 50 µL of CD4 monoclonal antibody (4H5) at the concentration as suggest in the APPLICATIONS diluted in the washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN3] into each tube.
2) Add 50 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at room temperature (20~25 oC).
3) Add 1 mL of washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
4) Add 30 µL of 1:100 FITC conjugated anti-mouse IgG diluted with washing buffer. Mix well and incubate for 15 minutes at room temperature.
5) Add 1 mL of washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
6) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (for analysis on BD instruments), using the procedure recommended in the respective package inserts.
7) Add 1 mL of H2O to each tube and incubate for 10 minutes at room temperature.
8) Centrifuge at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
9) Add 1 mL of washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
10) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.
(Positive controls for Flow cytometry; lymphocyte, monocyte)

General Readings
  1. Ravichandran, K. S., et al., Microbiol. Immunol. 205, 47-62 (1996).
  2. Leahy, D. J., FASEB J. 9, 17-25 (1995).
Storage

Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Protein A agarose
Buffer System:
PBS containing 50% glycerol, pH 7.2. No preservative is contained.
State:
Liquid Ig fraction
Azide Free
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
This antibody reacts with CD4 antigen.
Gene ID 920

Accessory Products

Proteins and/or Positive Controls

Proteins for CD4 (37 products)

Catalog No. Species Pres. Purity   Source  

CD4

CD4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-396, His-tag)

CD4 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

CD4 (26-396, His-tag)

CD4 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 90 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD4

CD4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4 (125-202, His-tagged)

CD4 Human Purified > 90 % E. coli
  Genesis Biotech Inc.

CD4 (203-317, His-tagged)

CD4 Human Purified > 90 % E. coli
  Genesis Biotech Inc.

CD4

CD4 structure and sequence Human Purified > 90 % Insect cells
2 µg / €190.00
  Neuromics Antibodies

CD4

CD4 structure and sequence Human Purified > 90 %
10 µg / €290.00
  Neuromics Antibodies

CD4 (1-150)

CD4 Human Liquid E. coli
  Neuromics Antibodies

CD4 (1-150)

CD4 Human Liquid E. coli
  Neuromics Antibodies

CD4 (26-226)

CD4 Human Purified E. coli
2 µg / €310.00
  Neuromics Antibodies

CD4 (26-226)

CD4 Human Purified E. coli
5 µg / €440.00
  Neuromics Antibodies
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