AM39008PU-N CD4 antibody

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1 ml / €240.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD4 Edu-2

Product Description for CD4

Mouse anti Human CD4 Edu-2.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Paraffin Sections, Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation Aff - Purified
Reactivity Hu
Applications C, F, P
Clonality Monoclonal
Clone Edu-2
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01730
GeneID:
920
Add. information 1. Conjugates with brighter fluorochromes, like PE and APC, will have a greater separation than those with dyes like FITC. When populations overlap, the percentage of positive cells using a selected marker can be affected by the choice of fluorescent label.
2. Use of monoclonal antibodies in patient treatment can interfere with antigen target recognition by this reagent. This should be taken into account when samples are analyzed from patients treated in this fashion.
3. Reagent data performance is based on EDTA-treated blood. Reagent performance can be affected by the use of other anticoagulants.
Application - Flow cytometry: The CD4 antibody (clone Edu-2) is used in routine blood testing for CD4+ cells and CD4/CD8 ratios (e.g. HIV/AIDS patients) or as part of panels for the detection and differentiation of certain T cell leukemias.
CD4 is also used in studies of functional activity of Th-cells in bacterial and viral infections, development of auto-immune diseases, transplant rejection, immune protection in response to allergens or allergenic reactivity (see "Protocols" below).
- Immunofluorescence / Immunohistochemistry using frozen and paraffin embedded tissue sections.
Background CD4 plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, this antigen acts as a receptor for HIV-1 by binding the viral protein gp120. The CD4 antigen is present on most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and weak on macrophages.
Concentration 0.1 mg / ml
Protocols Flow cytometry method for use with purified monoclonal antibodies
1. Add 100 µl of EDTA-treated blood (i.e. approx. 10e6 leukocytes) to a 5 ml reagent tube. The content of one tube is sufficient to perform one test.
2. Add to each tube 10 µl of purified monoclonal antibody. (Appropriate mouse Ig isotype control samples should always be included in any labeling study).
Vortex the tube to ensure thorough mixing of antibody and cells.
3. Incubate the tube for 15 minutes at room temperature in the dark.
4. Wash the labeled cells by adding 2 ml of PBS containing 0.001% (v/v) Heparin, vortexing and centrifuging (2 min 1000 x g) and discard the supernatant.
5. Add 50 µl of appropriate dilution of F(ab)2 Rabbit Anti Mouse IgG fluorescent conjugate (e.g.FITC or R-PE) in PBS containing 0.001% (v/v) Heparin to the tube. It is recommended that the tube is protected from light.
6. Mix by vortexing and incubate for 15 minutes at room temperature in the dark.
7. Add 100 µl of a lyse reagent and mix immediately.
8. Incubate for 10 minutes at room temperature in the dark.
9. Add 2 ml of demineralized water and incubate for 10 minutes in the dark.
10. Centrifuge the labeled cell suspension for 2 minutes at 1000 x g.
11. Remove the supernatant and resuspend the cells in 200 µl of PBS.
12. Analyze by flow cytometry within four hours (alternatively, the cells may be fixed by 0.05% of formaline in buffered saline for analysis the next day. Some antigens are readily destroyed upon fixation and this should be taken into account when using this alternative).
General Readings
  1. Carrière, D., et al., 1995, In: Leukocyte Typing V: 475-476. S.F. Schlossman, L. Boumsell, W., et al., eds. Oxford University Press, New York
  2. Piatier-Tonneau, D., et al.,1995, In: Leukocyte Typing V: 476-478. S.F. Schlossman, L. Boumsell, W. Gilks, et al., eds. Oxford University Press, New York
  3. Kaneoka H, Perez-Rojas G, Sasasuki T, Benike CJ, Engleman EG. Human T lymphocyte proliferation induced by a pan-T monoclonal antibody (anti-Leu 4): heterogeneity of response is a function of monocytes. J Immunol. 1983 Jul;131(1):158-64. PubMed PMID: 6408167.
  4. Lanier LL, Allison JP, Phillips JH. Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. J Immunol. 1986 Oct 15;137(8):2501-7. PubMed PMID: 3531334.
  5. Knowles, R.W., 1986. In: Leukocyte Typing II : 259-288; E.L. Reinhert, B.F. Haynes, L.M. Nadler/ and I.D. Bernstein, eds. Springer-Verlag, New York
  6. Friedrich W, O'Reilly RJ, Koziner B, Gebhard DF, Good RA, Evans RL. T-lymphocyte reconstitution in recipients of bone marrow transplants with and without GVHD: imbalances of T-cell subpopulations having unique regulatory and cognitive functions. Blood. 1982 Apr;59(4):696-701. PubMed PMID: 6800422.
  7. Allison JP, Lanier LL. Structure, function, and serology of the T-cell antigen receptor complex. Annu Rev Immunol. 1987;5:503-40. PubMed PMID: 2439105.
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2% BSA, 0.09% sodium azide
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
Anti CD4 (Edu-2) recognizes the CD4 antigen (a 55 kD glycoprotein).
Species:
Human.
Gene ID 920

Accessory Products

Proteins and/or Positive Controls

Proteins for CD4 (37 products)

Catalog No. Species Pres. Purity   Source  

CD4

CD4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-396, His-tag)

CD4 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

CD4 (26-396, His-tag)

CD4 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 90 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD4

CD4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4 (125-202, His-tagged)

CD4 Human Purified > 90 % E. coli
  Genesis Biotech Inc.

CD4 (203-317, His-tagged)

CD4 Human Purified > 90 % E. coli
  Genesis Biotech Inc.

CD4

CD4 structure and sequence Human Purified > 90 % Insect cells
2 µg / €190.00
  Neuromics Antibodies

CD4

CD4 structure and sequence Human Purified > 90 %
10 µg / €290.00
  Neuromics Antibodies

CD4 (1-150)

CD4 Human Liquid E. coli
  Neuromics Antibodies

CD4 (1-150)

CD4 Human Liquid E. coli
  Neuromics Antibodies

CD4 (26-226)

CD4 Human Purified E. coli
2 µg / €310.00
  Neuromics Antibodies

CD4 (26-226)

CD4 Human Purified E. coli
5 µg / €440.00
  Neuromics Antibodies
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