CL004APC CD4 antibody

See related secondary antibodies

Search for all "CD4"

0.1 mg / €530.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Mouse CD4 CT-CD4

Product Description for CD4

Rat anti Mouse CD4 CT-CD4.
Presentation: APC
Product is tested for Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation APC
Reactivity Ms
Applications F
Clonality Monoclonal
Clone CT-CD4
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P06332
GeneID:
12504
Application Flow Cytometry (see ''Protocols'' below).
(Reported to be useful in Immunohistochemistry on Acetone Fixed Frozen Sections).
Background Mouse CD4 (L3T4) which is expressed on the majority of thymocytes and on the MHC class II restricted subset of mature T cells including Th cells1,2. Mouse CD4 has also been reported to be present on multipotential hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic precursors2,3. As a coreceptor in the TCR complex, CD4 is involved in T cell activation through interaction with MHC class II on APC’s and in signal transduction via protein tyrosine kinase lck1.
The CD4 (L3/T4) antigen appears to be expressed by the helper/inducer subset of murine T cells and by delayed hypersensitivity T cells but not by cytotoxic T cells or their precursors. CD4 (L3/T4) and CD8a (Ly 2) have been shown to be present on mutually exclusive T cells in the peripheral lymphoid organs but the thymus contains cells expressing both CD4 (L3/T4) and CD8a (Ly 2).
Concentration 0.1 mg/ml (lot specific)
Protocols FLOW CYTOMETRY ANALYSIS:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add ~1 µg* of this Ab per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

MEDIA:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: BALB/c
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 1 µg/10e6 cells
Isotypic Control: APC Rat IgG2a
General Readings
  1. Bierer BE, Sleckman BP, Ratnofsky SE, Burakoff SJ. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989;7:579-99. PubMed PMID: 2653377.
  2. Frederickson GG, Basch RS. L3T4 antigen expression by hemopoietic precursor cells. J Exp Med. 1989 Apr 1;169(4):1473-8. PubMed PMID: 2784487. (Free PMC Article available)
  3. Wu L, Scollay R, Egerton M, Pearse M, Spangrude GJ, Shortman K. CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus. Nature. 1991 Jan 3;349(6304):71-4. PubMed PMID: 1702186.
  4. Palathumpat V, Holm B, Dejbakhsh-Jones S, Strober S. Treatment of BCL1 leukemia by transplantation of low density fractions of allogeneic bone marrow and spleen cells. J Immunol. 1992 May 15;148(10):3319-26. PubMed PMID: 1578152.
  5. Gross JA, Callas E, Allison JP. Identification and distribution of the costimulatory receptor CD28 in the mouse. J Immunol. 1992 Jul 15;149(2):380-8. PubMed PMID: 1320641.
  6. Darby CR, Hamano K, Wood KJ. Purification of monoclonal antibodies from tissue culture medium depleted of IgG. J Immunol Methods. 1993 Feb 26;159(1-2):125-9. PubMed PMID: 8445245.
  7. Darby CR, Morris PJ, Wood KJ. Evidence that long-term cardiac allograft survival induced by anti-CD4 monoclonal antibody does not require depletion of CD4+ T cells. Transplantation. 1992 Sep;54(3):483-90. PubMed PMID: 1384184.
  8. Cobbold SP, Jayasuriya A, Nash A, Prospero TD, Waldmann H. Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo. Nature. 1984 Dec 6-12;312(5994):548-51. PubMed PMID: 6150440.
  9. Agel, N.M. et al 1984 J. Immunol. Methods 69:207-214.
  10. Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51. PubMed PMID: 6415170.
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
APC
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
Stabilizers:
EIA grade BSA
State:
Liquid purified Ig fraction.
Specificity
Specificity:
This antibody detects Mouse CD4 (L3/T4). Other species not tested.
Gene ID 12504

Accessory Products

  • LinkedIn