CL004BX CD4 antibody

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0.3 mg / €610.00
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Quick Overview

Rat anti Mouse CD4 CT-CD4

Product Description for CD4

Rat anti Mouse CD4 CT-CD4.
Presentation: Biotin
Product is tested for Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 0.3 mg
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation Biotin
Reactivity Ms
Applications F
Clonality Monoclonal
Clone CT-CD4
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P06332
GeneID:
12504
Application Flow Cytometry (see Protocols).
(Reported to be useful in immunohistochemistry on acetone fixed frozen sections).
Background CD4 (L3T4) is expressed on the majority of thymocytes and on the MHC class II restricted subset of mature T cells including Th cells1,2. Mouse CD4 has also been reported to be present on multipotential hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic precursors2,3. As a coreceptor in the TCR complex, CD4 is involved in T cell activation through interaction with MHC class II on APC’s and in signal transduction via protein tyrosine kinase lck1.
Concentration 0.1 mg/ml
Protocols FLOW CYTOMETRY ANALYSIS:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test).
4. To each tube, add ~1.0-0.5 µg of this Ab per 106 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 µl of (Streptavidin-PE) at a 1:20 dilution.
9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C.
11. Resuspend the cell pellet in 50 µl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: BALB/c
Cell Concentration: 1x106 cells per test
Antibody Concentration Used: 0.5 µg/106 cells
Isotypic Control: Biotin Rat IgG2a
General Readings
  1. Bierer, B.E. et al. 1989. Annu. Rev. Immunol. 7: 579-599.
  2. Fredrickson, G.G. et al. 1989. J. Exp. Med. 169: 1473-1478.
  3. Wu, L. et al. 1991. Nature 349: 71-74. Dialynas, D.P. et al 1983 J. Immunol. 131:2445-2451.
  4. Palathumpat, V. et al. 1992 J. Immunol. 148:3319-3326.
  5. Gross, J. A. et al. 1992 J. Immunol. 149:380-388.
  6. Darby, C.R. et al. 1993 J. Immunol. 159:125-129.
  7. Darby, C.R. et al. 1992 Transplantation 54:483-490.
  8. Cobbold, S.P. et al. 1984 Nature 312:548-551.
  9. Agel, N.M. et al 1984 J. Immunol. Methods 69:207-214.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Biotin
Buffer System:
PBS containing 0.09% Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
State:
Liquid purified IgG fraction
Species Reactivity
Species reactivity (tested):
Mouse.
Specificity
Specificity:
This CT-CD4 monolconal antibody (mAb) recognizes mouse CD4 (L3T4).
Gene ID 12504

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