SM019FT CD4 (non-polymorphic epitope) antibody

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25 µg / €190.00
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Rat anti Mouse CD4 YTS177.9

Product Description for CD4

Rat anti Mouse CD4 YTS177.9.
Properties: (non-polymorphic epitope)
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 25 µg
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation FITC
Reactivity Ms
Applications F
Clonality Monoclonal
Clone YTS177.9
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P06332
Immunogen:
Mouse spleen cells.
Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
GeneID:
12504
Property non-polymorphic epitope
Application Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells or 100µl whole blood. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Background CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.
Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin
Intracellular ligands: p56Lck
CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Concentration 0.1 mg/ml
General Readings
  1. Qin, S. et al. (1990) Induction of tolerance in peripheral T-cells with monoclonal antibodies. Eur. J. Immunol. 20: 2737-2745.
  2. Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in MHC-mismatched or primed recipients: primed T-cells can be tolerised in the periphery with CD4 and CD8 antibodies. Eur. J. Immunol. 20: 2747-2755.
  3. Wise MP, Bemelman F, Cobbold SP, Waldmann H. Linked suppression of skin graft rejection can operate through indirect recognition. J Immunol. 1998 Dec 1;161(11):5813-6. PubMed PMID: 9834057.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity Chromatography on Protein G.
Buffer System:
PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
State:
Liquid purified IgG fraction.
Specificity
Specificity:
This antibody reacts with the CD4 antigen; non polymorphic epitope.
The antibody is reported to block MHC class II dependant T-cell responses in vitro and in vivo and induces tolerance.
Species:
Mouse.
Gene ID 12504

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