SM1663C CD4 antibody

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100 Tests / €320.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD4 7E14

Product Description for CD4

Mouse anti Human CD4 7E14.
Presentation: PE-Cy5
Product is tested for Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 100 Tests
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation PE-Cy5
Reactivity Hu
Applications F
Clonality Monoclonal
Clone 7E14
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01730
Immunogen:
CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
GeneID:
920
Add. information Cy-5 Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.
Application Flow Cytometry.
Monitoring of T cells subsets in peripheral blood.
Analysis of T cell subsets involved in helper/inducer functions.
Characterization of subtypes of T cell leukemias and lymphomas.
Studies of AIDS virus infection.
Background Identification of human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes.
Concentration Lot specific
Protocols PBMC:
Add 5 ul of Anitbody/10e6 PBMC in 100 ul PBS.
Mix gently and incubate for 15 minutes at 2-8°C.
Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
WHOLE BLOOD:
Add 5 ul of Antibody/100 ul of whole blood.
Mix gently and incubate for 15 minutes at room
temperature 20°C.
Lyse the whole blood.
Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a Flow cytometer or microscope.
General Readings
  1. Thymus Dependent Membrane Antigens in Man: Inhibition of Cell-Mediated Lympholysis by Monoclonal Antibodies to the TH-2 Antigen. Evans,R.L., Wall,D.W., Platsoucas,C.D., Siegal,F.P., Fikrig,S.M., Testa,C.M, and Good,R.A. Proc. Nat. Acad. Sci. 78,544,1981.
  2. Clement LT, Vink PE, Bradley GE. Novel immunoregulatory functions of phenotypically distinct subpopulations of CD4+ cells in the human neonate. J Immunol. 1990 Jul 1;145(1):102-8. PubMed PMID: 1694200.
  3. Antigen Presentation by the CD4 Positive Monocyte Subset. Szabo,G., Miller,C.L., Kodys,K., J. Leukoc. Biol. 47(2): 111-20,1990.
  4. Diamond DC, Finberg R, Chaudhuri S, Sleckman BP, Burakoff SJ. Human immunodeficiency virus infection is efficiently mediated by a glycolipid-anchored form of CD4. Proc Natl Acad Sci U S A. 1990 Jul;87(13):5001-5. PubMed PMID: 2142306. (Free PMC Article available)
  5. Adkins B. Developmental regulation of the intrathymic T cell precursor population. J Immunol. 1991 Mar 1;146(5):1387-93. PubMed PMID: 1899686.
  6. Induction of CD4 and Susceptibility to HIV-1 Infection in Human CD8+ T Lymphocytes by Human Herpesvirus 6. Lusso,P., De Maria,A., Malnati,M., Lori,F., DeRocco,S.E., Baseler,M., Gallo,R.C., Nat. 349(6309):533-5,1991.
  7. Shapiro HM, Glazer AN, Christenson L, Williams JM, Strom TB. Immunofluorescence measurement in a flow cytometer using low-power helium-neon laser excitation. Cytometry. 1983 Nov;4(3):276-9. PubMed PMID: 6363017.
  8. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987
Storage Store the antibody undiluted (protected from light) at 2-8°C
Do Not Freeze!
Shelf life: One year from despatch.
Format
Label:
PE-Cy5
Absorption emission:
488 nm / 670 nm
Buffer System:
PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
Purity:
PE-Cy5
State:
Liquid purified IgG fraction.
Specificity
Species:
Human.
Gene ID 920

Accessory Products

Proteins and/or Positive Controls

Proteins for CD4 (37 products)

Catalog No. Species Pres. Purity   Source  

CD4

CD4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-396, His-tag)

CD4 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

CD4 (26-396, His-tag)

CD4 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 90 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD4

CD4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4 (125-202, His-tagged)

CD4 Human Purified > 90 % E. coli
  Genesis Biotech Inc.

CD4 (203-317, His-tagged)

CD4 Human Purified > 90 % E. coli
  Genesis Biotech Inc.

CD4

CD4 structure and sequence Human Purified > 90 % Insect cells
2 µg / €190.00
  Neuromics Antibodies

CD4

CD4 structure and sequence Human Purified > 90 %
10 µg / €290.00
  Neuromics Antibodies

CD4 (1-150)

CD4 Human Liquid E. coli
  Neuromics Antibodies

CD4 (1-150)

CD4 Human Liquid E. coli
  Neuromics Antibodies

CD4 (26-226)

CD4 Human Purified E. coli
2 µg / €310.00
  Neuromics Antibodies

CD4 (26-226)

CD4 Human Purified E. coli
5 µg / €440.00
  Neuromics Antibodies
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