SM2271P CD4 antibody

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0.25 mg / €460.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Duck CD4 Du CD4-2

SM2271P

Product Description for CD4

Mouse anti Duck CD4 Du CD4-2.
Presentation: Purified
Product is tested for Immunoprecipitation, Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms T-Cell Surface Antigen T4/Leu-3, T-cell Surface Glycoprotein CD4, T4/Leu-3
Presentation Purified
Reactivity Duck
Applications F, IP
Clonality Monoclonal
Clone Du CD4-2
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q50EU7
Immunogen:
293T cells expressing Pekin duck CD4.
Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
Isotype control AM03096PU-N
Application Flow Cytometry.
Immunoprecipitation.
Background CD4 is shown to be expressed by thymocytes, splenocytes and peripheral lymphoid cells. Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs, the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA), and all cells reacting with clone Du CD4-2 have been identified as CD3+ T cells. (1)
Concentration 1.0 mg/ml
General Readings
  1. Kothlow S, Mannes NK, Schaerer B, Rebeski DE, Kaspers B, Schultz U. Characterization of duck leucocytes by monoclonal antibodies. Dev Comp Immunol. 2005;29(8):733-48. Epub 2005 Feb 8. PubMed PMID: 15854685.
  2. Higgins DA, Teoh CS. Duck lymphocytes. II. Culture conditions for optimum transformation response to phytohaemagglutinin. J Immunol Methods. 1988 Jan 21;106(1):135-45. PubMed PMID: 3339247.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G.
Buffer System:
PBS containing 0.09% Sodium Azide as preservative.
State:
Liquid purified IgG fraction.
Purified
Specificity
Specificity:
This antibody recognises CD4.
Clone Du CD4-2 can be used to identify duck T helper cells.
Species:
Duck. Does not react with Chicken.

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