SM255P CD4 (Domain 1) antibody

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0.25 mg / €430.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Rat CD4 W3/25


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  • SM255P

Product Description for CD4

Mouse anti Rat CD4 W3/25.
Properties: (Domain 1)
Presentation: Purified
Product is tested for Frozen Sections, Functional assay, Flow Cytometry, Paraffin Sections.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 0.25 mg
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation Purified
Reactivity Rt
Applications C, F, FN, P
Clonality Monoclonal
Clone W3/25
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Rat Thymocyte Membrane Glycoproteins.
Spleen cells from immunised BALB/c mice were fused with cells of the NS-1 mouse myeloma cell line.
Property Domain 1
Isotype control AM03095PU-N, SM20P (for use in rat samples)
Application Flow Cytometry: Use 10 μl of 1/100-1/200 diluted antibody to label 106 cells in 100 μl. 
Routinely tested on Rat splenocytes.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections: PLP fixation is recommended for optimal results, See Whiteland et al. for details.
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Concentration 1.0 mg/ml
General Readings
  1. Williams AF, Galfrè G, Milstein C. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 1977 Nov;12(3):663-73. PubMed PMID: 303545.
  2. Barclay AN. The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42(4):593-600. PubMed PMID: 7016746. (Free PMC Article available)
  3. Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 1995 Mar;43(3):313-20. PubMed PMID: 7868861.
  4. Pelegrí C, Franch A, Castellote C, Castell M. Immunohistochemical changes in synovial tissue during the course of adjuvant arthritis. J Rheumatol. 1995 Jan;22(1):124-32. PubMed PMID: 7535358.
  5. Hofmann N, Lachnit N, Streppel M, Witter B, Neiss WF, Guntinas-Lichius O, et al. Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats. BMC Immunol. 2002 Aug 26;3:11. PubMed PMID: 12196270. (Free PMC Article available, 5 images available)
  6. Zilka N, Stozicka Z, Kovac A, Pilipcinec E, Bugos O, Novak M. Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy. J Neuroimmunol. 2009 Apr 30;209(1-2):16-25. doi: 10.1016/j.jneuroim.2009.01.013. Epub 2009 Feb 20. PubMed PMID: 19232747.
  7. Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, et al. NK cell depletion delays corneal allograft rejection in baby rats. Mol Vis. 2010 Oct 2;16:1928-35. PubMed PMID: 21031017. (Free PMC Article available, 4 images available)
  8. Banerjee S, Figueiredo FC, Easty DL, Dick AD, Nicholls SM. Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats. Br J Ophthalmol. 2003 Dec;87(12):1515-22. PubMed PMID: 14660464. (Free PMC Article available, 6 images available)
  9. Bjersing JL, Telemo E, Dahlgren U, Hanson LA. Loss of ileal IgA+ plasma cells and of CD4+ lymphocytes in ileal Peyer's patches of vitamin A deficient rats. Clin Exp Immunol. 2002 Dec;130(3):404-8. PubMed PMID: 12452829. (Free PMC Article available, 4 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G
Buffer System:
0.09% Sodium Azide
Liquid purified IgG fraction from Tissue Culture Supernatant
This antibody recognises the CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes.
This antibody inhibits proliferation and IL-2 production in the MLR reaction. 
This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde).

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