SM3019P CD4 antibody

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0.1 mg / €210.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human CD4 MEM-115

SM3019P

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  • SM3019P

Product Description for CD4

Mouse anti Human CD4 MEM-115.
Presentation: Purified
Product is tested for Functional assay, Immunoprecipitation, Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation Purified
Reactivity Hu
Applications F, FN, IP
Clonality Monoclonal
Clone MEM-115
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01730
Immunogen:
Human thymocytes and T lymphocytes
GeneID:
920
Isotype control AM03096PU-N
Application Immunoprecipitation.
Functional Application: The antibody blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions.
Flow Cytometry: Although it has not been tested rigorously, following data suggest that the antibody is a low-affinity antibody: its binding to T cells increases at elevated temperature; monovalent Fab fragments essentially do not bind to T cells.
Background CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.
Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin 
Intracellular ligands: p56Lck 
CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Concentration 1.0 mg/ml
General Readings
  1. Millán J, Cerny J, Horejsi V, Alonso MA. CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Tissue Antigens. 1999 Jan;53(1):33-40. PubMed PMID: 10082429.
  2. Foti M, Phelouzat MA, Holm A, Rasmusson BJ, Carpentier JL. p56Lck anchors CD4 to distinct microdomains on microvilli. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2008-13. PubMed PMID: 11854499. (Free PMC Article available, 5 images available)
  3. Clapham PR, McKnight A.: Cell surface receptors, virus entry and tropism of primate lentiviruses. J Gen Virol. 2002 Aug;83(Pt 8):1809-29.
  4. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  5. Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, et al. LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62. Epub 2003 Nov 10. PubMed PMID: 14610046. (Free PMC Article available, 9 images available)
  6. Bosze S, Caccamo N, Majer Z, Mezo G, Dieli F, Hudecz F. In vitro T-cell immunogenicity of oligopeptides derived from the region 92-110 of the 16-kDa protein of Mycobacterium tuberculosis. Biopolymers. 2004;76(6):467-76. PubMed PMID: 15386261.
  7. Singer II, Scott S, Kawka DW, Chin J, Daugherty BL, DeMartino JA, DiSalvo J, Gould SL, Lineberger JE, Malkowitz L, Miller MD, Mitnaul L, Siciliano SJ, Staruch MJ, Williams HR, Zweerink HJ, Springer MS: CCR5, CXCR4, and CD4 are clustered and closely apposed on microvilli of human macrophages and T cells.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Protein A Affinity Chromatography
Buffer System:
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
State:
Liquid purified Ig fraction (> 95% by SDS-PAGE)
Purified
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
The antibody MEM-115 recognizes an epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (helper T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.
Gene ID 920

Accessory Products

Proteins and/or Positive Controls

Proteins for CD4 (37 products)

Catalog No. Species Pres. Purity   Source  

CD4

CD4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-396, His-tag)

CD4 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

CD4 (26-396, His-tag)

CD4 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 90 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD4

CD4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4 (125-202, His-tagged)

CD4 Human Purified > 90 % E. coli
  Genesis Biotech Inc.

CD4 (203-317, His-tagged)

CD4 Human Purified > 90 % E. coli
  Genesis Biotech Inc.

CD4

CD4 structure and sequence Human Purified > 90 % Insect cells
2 µg / €190.00
  Neuromics Antibodies

CD4

CD4 structure and sequence Human Purified > 90 %
10 µg / €290.00
  Neuromics Antibodies

CD4 (1-150)

CD4 Human Liquid E. coli
  Neuromics Antibodies

CD4 (1-150)

CD4 Human Liquid E. coli
  Neuromics Antibodies

CD4 (26-226)

CD4 Human Purified E. coli
2 µg / €310.00
  Neuromics Antibodies

CD4 (26-226)

CD4 Human Purified E. coli
5 µg / €440.00
  Neuromics Antibodies
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