SM3020B CD4 (N-term) antibody

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0.1 mg / €260.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human CD4 MEM-241

SM3020B

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  • SM3020B
  • SM3020B

Product Description for CD4

Mouse anti Human CD4 MEM-241.
Properties: (N-term)
Presentation: Biotin
Product is tested for Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation Biotin
Reactivity Hu
Applications F
Clonality Monoclonal
Clone MEM-241
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01730
Immunogen:
2 N-terminal domains of human CD4 fused to human IgG1 Fc
GeneID:
920
Property N-term
Isotype control SM10B (for use in human samples)
Application Flow Cytometry (1/1500).
Background CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin
Intracellular ligands: p56Lck
CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Concentration 1.0 mg/ml
General Readings
1. Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Tissue Antigens. 1999 Jan;53(1):33-40.
2. Foti M, Phelouzat MA, Holm A, Rasmusson BJ, Carpentier JL: p56Lck anchors CD4 to distinct microdomains on microvilli. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2008-13.
Clapham PR, McKnight A: Cell surface receptors, virus entry and tropism of primate lentiviruses. J Gen Virol. 2002 Aug;83(Pt 8):1809-29.
3. Brdickova N. et al.: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.
4. Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X: CD molecules 2006--human cell differentiation molecules. J Immunol Methods. 2007 Jan 30;319(1-2):1-5.
5. Karlsson KR, Cowley S, Martinez FO, Shaw M, Minger SL, James W: Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3. Exp Hematol. 2008 Sep;36(9):1167-75.
6. Manasa J, Musabaike H, Masimirembwa C, Burke E, Luthy R, Mudzori J: Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe. Clin Vaccine Immunol. 2007 Mar;14(3):293-8.
7. Anderson AE, Sayers BL, Haniffa MA, Swan DJ, Diboll J, Wang XN, Isaacs JD, Hilkens CM: Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells. J Leukoc Biol. 2008 Jul;84(1):124-33.
8. Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 5;12:2.
9. Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61.
Storage Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Label:
Conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Biotin
Buffer System:
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
State:
Liquid purified Ig fraction
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
The antibody recognizes CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (helper T-cells) and also on monocytes, tissue macrophages and granulocytes.
Gene ID 920

Accessory Products

Proteins and/or Positive Controls

Proteins for CD4 (37 products)

Catalog No. Species Pres. Purity   Source  

CD4

CD4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified >90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (125-202)

CD4 Human Purified >90.0% as determined by SDS-PAGE. E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-226)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (203-317)

CD4 Human Purified E. coli
  Acris Antibodies GmbH

CD4 (26-396, His-tag)

CD4 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

CD4 (26-396, His-tag)

CD4 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 90 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Insect cells
  Acris Antibodies GmbH

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified
  Abnova Taiwan Corp.

CD4

CD4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD4

CD4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4

CD4 Human
  Abnova Taiwan Corp.

CD4 (125-202, His-tagged)

CD4 Human Purified > 90 % E. coli
  Genesis Biotech Inc.

CD4 (203-317, His-tagged)

CD4 Human Purified > 90 % E. coli
  Genesis Biotech Inc.

CD4

CD4 structure and sequence Human Purified > 90 % Insect cells
2 µg / €190.00
  Neuromics Antibodies

CD4

CD4 structure and sequence Human Purified > 90 %
10 µg / €290.00
  Neuromics Antibodies

CD4 (1-150)

CD4 Human Liquid E. coli
  Neuromics Antibodies

CD4 (1-150)

CD4 Human Liquid E. coli
  Neuromics Antibodies

CD4 (26-226)

CD4 Human Purified E. coli
2 µg / €310.00
  Neuromics Antibodies

CD4 (26-226)

CD4 Human Purified E. coli
5 µg / €440.00
  Neuromics Antibodies
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