SM409F CD4 antibody

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100 Tests / €640.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Canine CD4 YKIX302.9

Product Description for CD4

Rat anti Canine CD4 YKIX302.9.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 100 Tests
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation FITC
Reactivity Can
Applications F
Clonality Monoclonal
Clone YKIX302.9
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P33705
Immunogen:
Canine Concanavilin A activated T-cell blasts.
GeneID:
403931
Remarks:
Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Application Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells or 100 µl whole blood.
Background In the dog CD4 is expressed by neutrophils as well as by lymphocytes subsets.
Concentration 0.1 mg/ml
General Readings
  1. Cobbold S, Metcalfe S. Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994 Mar;43(3):137-54. PubMed PMID: 8091414.
  2. Gorman SD, Frewin MR, Cobbold SP, Waldmann H. Isolation and expression of cDNA encoding the canine CD4 and CD8 alpha antigens. Tissue Antigens. 1994 Mar;43(3):184-8. PubMed PMID: 8091416.
  3. Watson CJ, Cobbold SP, Davies HS, Rebello PR, Waldmann H, Calne RY, et al. CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog. Br J Surg. 1993 Nov;80(11):1389-92. PubMed PMID: 8252344.
  4. Papadogiannakis EI, Kontos VI, Tamamidou M, Roumeliotou A. Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture. Can J Vet Res. 2009 Apr;73(2):137-43. PubMed PMID: 19436583. (Free PMC Article available, 7 images available)
  5. Bauer TR, Hai M, Tuschong LM, Burkholder TH, Gu YC, Sokolic RA, et al. Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy. Blood. 2006 Nov 15;108(10):3313-20. Epub 2006 Jul 25. PubMed PMID: 16868255. (Free PMC Article available, 7 images available)
  6. Boggiatto PM, Ramer-Tait AE, Metz K, Kramer EE, Gibson-Corley K, Mullin K, et al. Immunologic indicators of clinical progression during canine Leishmania infantum infection. Clin Vaccine Immunol. 2010 Feb;17(2):267-73. doi: 10.1128/CVI.00456-09. Epub 2009 Dec 23. PubMed PMID: 20032217. (Free PMC Article available, 3 images available)
  7. Araújo MS, de Andrade RA, Sathler-Avelar R, Magalhães CP, Carvalho AT, Andrade MC, et al. Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis. Vet Immunol Immunopathol. 2011 May 15;141(1-2):64-75. doi: 10.1016/j.vetimm.2011.02.007. Epub 2011 Feb 23. PubMed PMID: 21439654.
  8. Benyacoub J, Czarnecki-Maulden GL, Cavadini C, Sauthier T, Anderson RE, Schiffrin EJ, et al. Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs. J Nutr. 2003 Apr;133(4):1158-62. PubMed PMID: 12672936.
  9. Bund D, Buhmann R, Gökmen F, Kremser A, Dreyssig J, Kolb HJ, et al. Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies. Cell Immunol. 2010;263(1):88-98. doi: 10.1016/j.cellimm.2010.03.003. Epub 2010 Mar 6. PubMed PMID: 20347071.
  10. Estrela-Lima A, Araújo MS, Costa-Neto JM, Teixeira-Carvalho A, Barrouin-Melo SM, Cardoso SV, et al. Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates. BMC Cancer. 2010 Jun 4;10:256. doi: 10.1186/1471-2407-10-256. PubMed PMID: 20525350. (Free PMC Article available, 6 images available)
  11. Out TA, Wang SZ, Rudolph K, Bice DE. Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs. Immunology. 2002 Apr;105(4):499-508. PubMed PMID: 11985670. (Free PMC Article available, 7 images available)
  12. Pinheiro D, Singh Y, Grant CR, Appleton RC, Sacchini F, Walker KR, et al. Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog. Immunology. 2011 Jan;132(1):111-22. doi: 10.1111/j.1365-2567.2010.03346.x. Epub 2010 Sep 30. PubMed PMID: 20880379. (Free PMC Article available, 3 images available)
  13. Mitchell L, Thamm DH, Biller BJ. Clinical and immunomodulatory effects of toceranib combined with low-dose cyclophosphamide in dogs with cancer. J Vet Intern Med. 2012 Mar-Apr;26(2):355-62. doi: 10.1111/j.1939-1676.2011.00883.x. Epub 2012 Feb 4. PubMed PMID: 22303814.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid purified IgG fraction
Specificity
Specificity:
This antibody Clone YKIX302.9 recognises the Canine CD4 cell surface antigen.
The antibody has been shown to block MHCII dependent T cell responses in vivo and in vitro. In the Dog CD4 is expressed by neutrophils as well as by lymphocytes subsets.
Species:
Canine (Dog).
Gene ID 403931

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