SM497F CD4 antibody

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0.1 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Equine CD4 CVS4

Product Description for CD4

Mouse anti Equine CD4 CVS4.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD4

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Presentation FITC
Reactivity Eq
Applications F
Clonality Monoclonal
Clone CVS4
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
F6Y6X8
Immunogen:
Equine thymocytes
Remarks:
Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Isotype control AM03095PU-N
Application

Flow cytometry: Neat-1/10, use 10µl of this working dilution to label 10e6 cells in 100µl.

Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Concentration 0.1 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Goodman LB, Loregian A, Perkins GA, Nugent J, Buckles EL, Mercorelli B, et al. A point mutation in a herpesvirus polymerase determines neuropathogenicity. PLoS Pathog. 2007 Nov;3(11):e160. PubMed PMID: 17997600. (Free PMC Article available, 7 images available)
  2. Go YY, Zhang J, Timoney PJ, Cook RF, Horohov DW, Balasuriya UB. Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes. J Virol. 2010 May;84(10):4898-911. doi: 10.1128/JVI.02743-09. Epub 2010 Mar 10. PubMed PMID: 20219931. (Free PMC Article available, 7 images available)
General Readings
  1. Lunn DP, Holmes MA, Duffus WP. Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets. Immunology. 1991 Oct;74(2):251-7. PubMed PMID: 1748472. (Free PMC Article available)
  2. Kydd, J.H. and Antczak, D.F., (1991) First International Workshop on Equine Leucocyte Antigens. Equine Immunol. 4 - 5.
  3. Deeg CA, Reese S, Gerhards H, Wildner G, Kaspers B. The uveitogenic potential of retinal S-antigen in horses. Invest Ophthalmol Vis Sci. 2004 Jul;45(7):2286-92. PubMed PMID: 15223807.
  4. Pearson W, Omar S, Clarke AF. Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses. Can J Vet Res. 2007 Jul;71(3):213-7. PubMed PMID: 17695597. (Free PMC Article available, 3 images available)
  5. Brault SA, Blanchard MT, Gardner IA, Stott JL, Pusterla N, Mapes SM, et al. The immune response of foals to natural infection with equid herpesvirus-2 and its association with febrile illness. Vet Immunol Immunopathol. 2010 Sep 15;137(1-2):136-41. doi: 10.1016/j.vetimm.2010.05.010. Epub 2010 Jun 1. PubMed PMID: 20646766.
  6. Hamza E, Steinbach F, Marti E. CD4+CD25+ T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity. Vet Immunol Immunopathol. 2012 Jul 15;148(1-2):139-44. doi: 10.1016/j.vetimm.2011.05.033. Epub 2011 Jun 6. PubMed PMID: 21700344.
  7. Lunn DP, Holmes MA, Antczak DF, Agerwal N, Baker J, Bendali-Ahcene S, et al. Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 1998 Mar 31;62(2):101-43. PubMed PMID: 9638857.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity chromatography on Protein G from tissue culture supernatant
Buffer System:
Containing 0.09% Sodium azide and 1% BSA
State:
Liquid purified IgG
Purified
Specificity
Specificity:
This anitbody reacts with T-helper cells, it recognises a 58kDa cell surface glycoprotein that is primarily expressed on a subpopulation of equine T lymphocytes and considered to be the equine homologue of human CD4.
Species:
Equine (Horse).

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