discontinued

SM606 CD43 / Leukosialin antibody

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2 ml / €540.00

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Mouse anti Rabbit CD43 / Leukosialin L11-135

SM606

Product Description for CD43 / Leukosialin

Mouse anti Rabbit CD43 / Leukosialin L11-135.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.

Properties for CD43 / Leukosialin

Product Category Primary Antibodies
Quantity 2 ml
Synonyms CD43, GALGP, Galactoglycoprotein, Leukocyte sialoglycoprotein, Leukosialin, SPN, Sialophorin
Presentation Supernatant
Reactivity Rb
Applications C, F, IP, WB
Clonality Monoclonal
Clone L11-135
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Glycoproteins from the RL-5 T-cell line. Spleen cells from immunised BALB/c mice were fused with cells of the P3.X63.Ag8-U1 mouse myeloma cell line.
Application Flow Cytometry.
Immunoprecipitation.
Immunohistochemistry on frozen sections.
Background CD43 is one of the major glycoproteins of thymocytes and T lymphocytes, it plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response.
CD43 is expressed by the majority of leucocytes, with the exception of most B cells. However CD43 expression is seen in some cases of B cell lymphocytic lymphoma and centrocytic lymphoma.
Concentration 16.7 mg/ml
General Readings
  1. Jackson S, Chused TM, Wilkinson JM, Leiserson WM, Kindt TJ. Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry. J Exp Med. 1983 Jan 1;157(1):34-46. PubMed PMID: 6600269. (Free PMC Article available)
  2. Wilkinson JM, Wetterskog DL, Sogn JA, Kindt TJ. Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies. Mol Immunol. 1984 Jan;21(1):95-103. PubMed PMID: 6200770.
  3. Wilkinson JM, Galea-Lauri J, Sellars RA, Boniface C. Identification and tissue distribution of rabbit leucocyte antigens recognized by monoclonal antibodies. Immunology. 1992 Aug;76(4):625-30. PubMed PMID: 1398751. (Free PMC Article available)
  4. Wilkinson JM, McDonald G, Smith S, Galea-Lauri J, Lewthwaite J, Henderson B, et al. Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit. J Pathol. 1993 Jul;170(3):315-20. PubMed PMID: 8133406.
  5. Okabayashi, M. et al. (1993) Shope papilloma cell and leucocyte proliferation in regressing and progressing lesions. Am. J. Pathol. 142 No. 2: 489-496.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
Containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum and 0.09% Sodium Azide
State:
Liquid Tissue Culture Supernatant
Supernatant
Specificity
Specificity:
This antibody recognises all T cells by flow cytometry; monocytes and macrophages stain weakly. Good staining of TCell dependent areas in lymphoid tissue sections is observed. This antibody immunoprecipitates and immunoblots a 120kD protein from RL-5 cells and T lymphocytes. It is believed to recognise the homologue of Human CD43, Leukosialin. Clone L11/135 has been reported as being suitable for use in Western blotting.

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