SM2030P CD45 (CD45R) antibody

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0.25 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Sheep CD45 20.96


Product Description for CD45

Mouse anti Sheep CD45 20.96.
Properties: (CD45R)
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for CD45

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms CD45R, L-CA, Leukocyte common antigen, PTPRC, T200
Presentation Purified
Reactivity Sh
Applications C, F, IP
Clonality Monoclonal
Clone 20.96
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Ovine thymocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Property CD45R
Application Immunoprecipitation.
Immunohistochemistry on frozen sections.
Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 1 x 10e6 cells in 100µl.
Background CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Concentration 1.0 mg/ml
General Readings
  1. Mackay CR, Maddox JF, Brandon MR. A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell Immunol. 1987 Nov;110(1):46-55. PubMed PMID: 2445494.
  2. Sainte Marie, G. et al. (1962) A paraffin embedding technique for studies employing immunofluorescence. J.Histochem. Cytochem. 10: 250
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide
Liquid purified IgG
This antibody recognises the CD45R cell surface antigen. In immunohistochemical staining the antibody labels all lymphocytes within the B cell follicles as well as scattered cells in interfollicular areas. This clone has been reported to react with paraffin-embedded material following alcohol fixation. Please refer to ref. Sainte Marie, et al for alcohol fixation protocol.

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