SM416R CD45 (CD45R) antibody

See related secondary antibodies

Search for all "CD45"

100 Tests / €590.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Canine CD45 YKIX753.22.2

SM416R

Product Description for CD45

Rat anti Canine CD45 YKIX753.22.2.
Properties: (CD45R)
Presentation: PE
Product is tested for Flow Cytometry.

Properties for CD45

Product Category Primary Antibodies
Quantity 100 Tests
Synonyms CD45R, L-CA, Leukocyte common antigen, PTPRC, T200
Presentation PE
Reactivity Can
Applications F
Clonality Monoclonal
Clone YKIX753.22.2
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Canine thymocytes. Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Property CD45R
Application Flow Cytometry.
Background CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
General Readings
  1. Cobbold S, Metcalfe S. Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994 Mar;43(3):137-54. PubMed PMID: 8091414.
Storage Prior to and following reconstitution store the antibody at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
R. Phycoerythrin (RPE)
PE
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
State:
Lyophilized purified IgG
Reconstitution:
Restore with 1ml distilled water
Specificity
Specificity:
This antibody was clustered as a CD45R at the first canine Leucocyte Antigen workshop. It precipitates an antigen of 220kD from Con A Blasts and recognises a CD45R isoform. It is expressed on subsets of leucocytes (neutrophils are weak) in canine blood of all our bred mongrels and beagles tested.
Species:
Dog.

Accessory Products

  • LinkedIn