SM467 CD45 antibody

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Mouse anti Sheep CD45 VPM18


Product Description for CD45

Mouse anti Sheep CD45 VPM18.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for CD45

Product Category Primary Antibodies
Quantity 2 ml
Synonyms CD45R, CD45RA, CD45RB, CD45RO, L-CA, Leukocyte common antigen, PTPRC, T200
Presentation Supernatant
Reactivity Sh
Applications C, F, IP
Clonality Monoclonal
Clone VPM18
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Sheep T lymphocytes.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO myeloma cell line.
Application Flow Cytometry: use 10 µl of neat antibody to label 10e6 cells or 100 µl whole blood.
Immunohistochemistry on Frozen Sections.
Background CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
General Readings
  1. Hopkins J, Dutia BM. Monoclonal antibodies to the sheep analogues of human CD45 (leucocyte common antigen), MHC class I and CD5. Differential expression after lymphocyte activation in vivo. Vet Immunol Immunopathol. 1990 Apr;24(4):331-46. PubMed PMID: 1692651.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Liquid Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% Foetal Calf Serum with 0.09% Sodium Azide as preservative.
This antibody recognises the ovine homologue of human CD45 (leucocyte common antigen).
The antibody recognises all isoforms of the molecule.
Does not cross react with human.

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