SM582F CD45 antibody

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0.1 mg / €430.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Guinea Pig CD45 IH-1

SM582F

Product Description for CD45

Mouse anti Guinea Pig CD45 IH-1.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD45

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CD45R, CD45RA, CD45RB, CD45RO, L-CA, Leukocyte common antigen, PTPRC, T200
Presentation FITC
Reactivity GP
Applications F
Clonality Monoclonal
Clone IH-1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Guinea pig leukemic B lymphocyte L2C cells
Add. information Immunoprecipitates proteins of M.Wt. 220,000, 210,000, 195,000 and 180,000 from lymph node lymphocytes and proteins of M.Wt 195,000, 180,000 and 170,000 from thymocytes.
Application Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 106 cells in 100 µl.
Background CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Concentration 0.1 mg/ml
General Readings
  1. Hart, I.J. et al. (1992) Subpopulations of guinea pig T lymphocytes defined by isoforms of the leucocytecommon antigen. Immunol. 77: 377-384. 
  2. Wang Y, Nagarajan U, Hennings L, Bowlin AK, Rank RG. Local host response to chlamydial urethral infection in male guinea pigs. Infect Immun. 2010 Apr;78(4):1670-81. doi: 10.1128/IAI.01339-09. Epub 2010 Feb 1. PubMed PMID: 20123720. (Free PMC Article available, 10 images available)
  3. Shang, S. et al. (2010) The activity of TMC 207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs. Antimicrob Agents Chemother. 2010 Oct 11. [Epub ahead of print]
  4. Lacy HM, Bowlin AK, Hennings L, Scurlock AM, Nagarajan UM, Rank RG. Essential role for neutrophils in pathogenesis and adaptive immunity in Chlamydia caviae ocular infections. Infect Immun. 2011 May;79(5):1889-97. doi: 10.1128/IAI.01257-10. Epub 2011 Mar 14. PubMed PMID: 21402767. (Free PMC Article available, 6 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light. 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS, pH 8.0
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid purified IgG fraction
Specificity
Specificity:
The antibody reacts with CD45 on all leucocytes.
Species:
Guinea Pig.

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