SM582P CD45 antibody

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0.2 mg / €470.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Guinea Pig CD45 IH-1


Product Description for CD45

Mouse anti Guinea Pig CD45 IH-1.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for CD45

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms CD45R, CD45RA, CD45RB, CD45RO, L-CA, Leukocyte common antigen, PTPRC, T200
Presentation Purified
Reactivity GP
Applications C, F, IP
Clonality Monoclonal
Clone IH-1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Isotype control AM03095PU-N
Application Flow Cytometry.
Immunohistochemistry on frozen sections.
Immunoprecipitation: Immunoprecipitates proteins of M.Wt. 220,000, 210,000 and 195,000 and 180,000 from lymph node lymphocytes and proteins of M.Wt 195,000, 180,000 and 170,000 from thymocytes.
Background CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Concentration 1mg/ml
General Readings 1. Hart I. J., Schafer H, Scheper, R.J. and Stevenson G.T. (1992) Subpopulations of guinea pig T lymphocytes defined by isoforms of the leucocyte common antigen. Immunol. 77:
377 - 384.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity chromatography on Protein A
Buffer System:
TRIS buffered saline, pH 8.0 containing 0.09% Sodium Azide
Liquid purified IgG
This antibody reacts with CD45 on all leucocytes.
Guinea Pig.

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