discontinued

SM608 CD45 antibody

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2 ml / €390.00

Quick Overview

Mouse anti Rabbit CD45 L12-201

SM608

Product Description for CD45

Mouse anti Rabbit CD45 L12-201.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for CD45

Product Category Primary Antibodies
Quantity 2 ml
Synonyms CD45R, CD45RA, CD45RB, CD45RO, L-CA, Leukocyte common antigen, PTPRC, T200
Presentation Supernatant
Reactivity Rb
Applications C, F, IP
Clonality Monoclonal
Clone L12-201
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Glycoproteins isolated from the T cell line, RL-5. Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8-U1 mouse myeloma cell line.
Application Flow cytometry (neat - 1/10): use 10 µl of the suggested working dilution to label 10e6 cells or cells or 100µl whole blood.
Immunohistochemistry on frozen sections.
Immunoprecipitation was only obtained after cross linking antibody to the labelled cell surface. This gave a band of 200 kDa.
Background CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
General Readings
  1. Jackson S, Chused TM, Wilkinson JM, Leiserson WM, Kindt TJ. Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry. J Exp Med. 1983 Jan 1;157(1):34-46. PubMed PMID: 6600269. (Free PMC Article available)
  2. Wilkinson JM, Wetterskog DL, Sogn JA, Kindt TJ. Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies. Mol Immunol. 1984 Jan;21(1):95-103. PubMed PMID: 6200770.
  3. Wilkinson JM, Galea-Lauri J, Reid HW. A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens. Immunology. 1992 Sep;77(1):106-8. PubMed PMID: 1328042. (Free PMC Article available)
  4. Wilkinson JM, McDonald G, Smith S, Galea-Lauri J, Lewthwaite J, Henderson B, et al. Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit. J Pathol. 1993 Jul;170(3):315-20. PubMed PMID: 8133406.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
0.2M Tris/HCL, pH 7.4, containing 0.09% Sodium Azide
State:
Liquid tissue culture supernatant
Supernatant
Specificity
Specificity:
This antibody shows pan leucocyte reactivity by flow cytometry and immunohistochemistry.
Species:
Rabbit.

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