DM3040 CD45RB antibody

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Mouse anti Human CD45RB DFB-1


Product Description for CD45RB

Mouse anti Human CD45RB DFB-1.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for CD45RB

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Hu
Applications C, F, P, WB
Clonality Monoclonal
Clone DFB-1
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Human spleen cells affected with hairy cell leukaemia.
Isotype control AM03097PU-N
Application Immunofluorescence: 5-20 µg/mL. Flow cytometry: 1-5 µg/106 cells. Immunoblotting: 1-5 µg/mL. Immunohistochemistry: 5-20 µg/mL on frozen sections using avidin-biotin system. The optimal dilution factors should be determined by the individual laboratory. This product was not quality controlled in flow cytometry. This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-10 mg/mL with the Auto/Probe III kit. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.4 mg/ml
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution
This antibody (DFB1) recognizes a 220 kD single chain glycoprotein. The antigen is expressed on naive T-cells and mature B-cells.

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