SM410F CD5 antibody

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0.1 mg / €520.00
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Quick Overview

Rat anti Canine CD5 YKIX322.3

Product Description for CD5

Rat anti Canine CD5 YKIX322.3.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD5

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms LEU-1, LEU1, Ly-1, Lymphocyte antigen T1/Leu-1, Lyt-1, T-cell surface glycoprotein CD5
Presentation FITC
Reactivity Can
Applications F
Clonality Monoclonal
Clone YKIX322.3
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Concanavilin A activated canine peripheral blood cells. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Isotype control SM15F, SM15FX
Application Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells or 100µl of whole blood.
Background CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
Concentration 0.1 mg/ml
General Readings
  1. Cobbold S, Metcalfe S. Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994 Mar;43(3):137-54. PubMed PMID: 8091414.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
State:
Liquid purified IgG
Specificity
Specificity:
This antibody recognises a 67kD cell surface glycoprotein expressed on the surface of T cells. Clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop.
Species:
Dog.

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