SM410P CD5 antibody

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Rat anti Canine CD5 YKIX322.3


Product Description for CD5

Rat anti Canine CD5 YKIX322.3.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for CD5

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms LEU-1, LEU1, Ly-1, Lymphocyte antigen T1/Leu-1, Lyt-1, T-cell surface glycoprotein CD5
Presentation Purified
Reactivity Can
Applications C, F, IP
Clonality Monoclonal
Clone YKIX322.3
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Concanavilin A activated canine peripheral blood cells. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Isotype control SM15P, SM15PX
Application Immunoprecipitation.
Immunohistochemistry on frozen sections.
Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Background CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
Concentration 1.0 mg/ml
General Readings
  1. Hewicker-Trautwein M, Carter SD, Bennett D, Kelly DF. Immunocytochemical demonstration of lymphocyte subsets and MHC class II antigen expression in synovial membranes from dogs with rheumatoid arthritis and degenerative joint disease. Vet Immunol Immunopathol. 1999 Mar 1;67(4):341-57. PubMed PMID: 10206202.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide
Liquid purified IgG
This antibody recognises a 67kD cell surface glycoprotein expressed on the surface of T cells. Clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop.

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