SM2135R CD52 / CAMPATH-1 antibody

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100 Tests / €820.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Human, Monkey, Rhesus Monkey CD52 / CAMPATH-1 YTH34.5

Product Description for CD52 / CAMPATH-1

Rat anti Human, Monkey, Rhesus Monkey CD52 / CAMPATH-1 YTH34.5.
Presentation: PE
Product is tested for Flow Cytometry.

Properties for CD52 / CAMPATH-1

Product Category Primary Antibodies
Target Category
Quantity 100 Tests
Synonyms CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5
Presentation PE
Reactivity Hu, Mky, Rhesus Mky
Applications F
Clonality Monoclonal
Clone YTH34.5
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P31358
Immunogen:
Human lymphocytes.
GeneID:
1043
Isotype control SM19R
Application Flow Cytometry: Use 10 µl of neat antibody to label 1 x 10e6 cells.
Background CD52 is a small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link (Xia et al. 1991). The apparent molecular mass of the native antigen on SDS-PAGE is 25-29kDa, considerably reduced following N-glycanase treatment (Rowan et al. 1998). CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies (Dearden et al. 2002; Pettitt et al. 2012).
General Readings
  1. Hale G, Zhang MJ, Bunjes D, Prentice HG, Spence D, Horowitz MM, et al. Improving the outcome of bone marrow transplantation by using CD52 monoclonal antibodies to prevent graft-versus-host disease and graft rejection. Blood. 1998 Dec 15;92(12):4581-90. PubMed PMID: 9845524.
  2. Salisbury JR, Rapson NT, Codd JD, Rogers MV, Nethersell AB. Immunohistochemical analysis of CDw52 antigen expression in non-Hodgkin's lymphomas. J Clin Pathol. 1994 Apr;47(4):313-7. PubMed PMID: 8027367. (Free PMC Article available)
  3. Rodig SJ, Abramson JS, Pinkus GS, Treon SP, Dorfman DM, Dong HY, et al. Heterogeneous CD52 expression among hematologic neoplasms: implications for the use of alemtuzumab (CAMPATH-1H). Clin Cancer Res. 2006 Dec 1;12(23):7174-9. PubMed PMID: 17145843.
  4. Haniffa M, Ginhoux F, Wang XN, Bigley V, Abel M, Dimmick I, et al. Differential rates of replacement of human dermal dendritic cells and macrophages during hematopoietic stem cell transplantation. J Exp Med. 2009 Feb 16;206(2):371-85. doi: 10.1084/jem.20081633. Epub 2009 Jan 26. PubMed PMID: 19171766. (Free PMC Article available, 7 images available)
  5. Ratzinger G, Reagan JL, Heller G, Busam KJ, Young JW. Differential CD52 expression by distinct myeloid dendritic cell subsets: implications for alemtuzumab activity at the level of antigen presentation in allogeneic graft-host interactions in transplantation. Blood. 2003 Feb 15;101(4):1422-9. Epub 2002 Oct 10. PubMed PMID: 12393688.
  6. Hu Y, Turner MJ, Shields J, Gale MS, Hutto E, Roberts BL, et al. Investigation of the mechanism of action of alemtuzumab in a human CD52 transgenic mouse model. Immunology. 2009 Oct;128(2):260-70. doi: 10.1111/j.1365-2567.2009.03115.x. PubMed PMID: 19740383. (Free PMC Article available, 9 images available)
  7. Golay J, Cortiana C, Manganini M, Cazzaniga G, Salvi A, Spinelli O, et al. The sensitivity of acute lymphoblastic leukemia cells carrying the t(12;21) translocation to campath-1H-mediated cell lysis. Haematologica. 2006 Mar;91(3):322-30. PubMed PMID: 16531255.
  8. Klangsinsirikul P, Carter GI, Byrne JL, Hale G, Russell NH. Campath-1G causes rapid depletion of circulating host dendritic cells (DCs) before allogeneic transplantation but does not delay donor DC reconstitution. Blood. 2002 Apr 1;99(7):2586-91. PubMed PMID: 11895797.
  9. Piccaluga PP, Agostinelli C, Righi S, Zinzani PL, Pileri SA. Expression of CD52 in peripheral T-cell lymphoma. Haematologica. 2007 Apr;92(4):566-7. PubMed PMID: 17488672.
  10. Gopcsa L, Banyai A, Jakab K, Kormos L, Tamaska J, Matolcsy A, et al. Extensive flow cytometric characterization of plasmacytoid dendritic cell leukemia cells. Eur J Haematol. 2005 Oct;75(4):346-51. PubMed PMID: 16146542.
  11. Chang ST, Lu CL, Chuang SS. CD52 expression in non-mycotic T- and NK/T-cell lymphomas. Leuk Lymphoma. 2007 Jan;48(1):117-21. PubMed PMID: 17325855.
  12. Westermann J, Maschmeyer G, van Lessen A, Dörken B, Pezzutto A. CD52 is not a promising immunotherapy target for most patients with multiple myeloma. Int J Hematol. 2005 Oct;82(3):248-50. PubMed PMID: 16207599.
  13. Reimer P, Rüdiger T, Geissinger E, Weissinger F, Nerl C, Schmitz N, et al. Autologous stem-cell transplantation as first-line therapy in peripheral T-cell lymphomas: results of a prospective multicenter study. J Clin Oncol. 2009 Jan 1;27(1):106-13. doi: 10.1200/JCO.2008.17.4870. Epub 2008 Nov 24. PubMed PMID: 19029417.
  14. Zand MS, Bose A, Vo T, Coppage M, Pellegrin T, Arend L, et al. A renewable source of donor cells for repetitive monitoring of T- and B-cell alloreactivity. Am J Transplant. 2005 Jan;5(1):76-86. PubMed PMID: 15636614.
  15. Rizzo K, Stetler-Stevenson M, Wilson W, Yuan CM. Novel CD19 expression in a peripheral T cell lymphoma: A flow cytometry case report with morphologic correlation. Cytometry B Clin Cytom. 2009 Mar;76(2):142-9. doi: 10.1002/cyto.b.20442. PubMed PMID: 18671252. (Free PMC Article available, 3 images available)
  16. Miles RR, Cairo MS, Satwani P, Zwick DL, Lones MA, Sposto R, et al. Immunophenotypic identification of possible therapeutic targets in paediatric non-Hodgkin lymphomas: a children's oncology group report. Br J Haematol. 2007 Aug;138(4):506-12. PubMed PMID: 17659054.
  17. Bisig, B. et al. (2013) Molecular and phenotypic features are shared by CD30-positive peripheral T-cell lymphomas Haematologica May 28. [Epub ahead of print]
Storage Prior to and following reconstitution store the antibody at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
R. Phycoerythrin (RPE)
PE
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA
State:
Lyophilized purified Ig fraction from Tissue Culture Supernatant
Reconstitution:
Restore with 1 ml distilled water.
Specificity
Specificity:
This antibody CD52 antibody, clone YTH34.5 reacts with the Human CD52 antigen, also known as CAMPATH-1.
Species:
Human, Rhesus Monkey.
Gene ID 1043

Accessory Products

Proteins and/or Positive Controls

Proteins for CD52 / CAMPATH-1 (1 products)

Catalog No. Species Pres. Purity   Source  

CD52 / CAMPATH-1

CD52 / CAMPATH-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.
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