SM3081P CD52 / CAMPATH-1 antibody

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0.1 mg / €230.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human CD52 / CAMPATH-1 HI186

SM3081P

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  • SM3081P

Product Description for CD52 / CAMPATH-1

Mouse anti Human CD52 / CAMPATH-1 HI186.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Paraffin Sections.

Properties for CD52 / CAMPATH-1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5
Presentation Purified
Reactivity Hu
Applications C, F, P
Clonality Monoclonal
Clone HI186
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P31358
Immunogen:
Human tonsil
GeneID:
1043
Isotype control AM03110PU-N, SM12P
Application Flow Cytometry: 2 µg/ml.
Immunohistochemistry on Frozen Sections.  
Immunohistochemistry on Paraffin-Embedded Sections.
Background CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.
Concentration 1.0 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
General Readings
  1. Treumann A, Lifely MR, Schneider P, Ferguson MA. Primary structure of CD52. J Biol Chem. 1995 Mar 17;270(11):6088-99. PubMed PMID: 7890742.
  2. Rowan WC, Hale G, Tite JP, Brett SJ. Cross-linking of the CAMPATH-1 antigen (CD52) triggers activation of normal human T lymphocytes. Int Immunol. 1995 Jan;7(1):69-77. PubMed PMID: 7718516.
  3. Schröter S, Derr P, Conradt HS, Nimtz M, Hale G, Kirchhoff C. Male-specific modification of human CD52. J Biol Chem. 1999 Oct 15;274(42):29862-73. PubMed PMID: 10514467.
  4. Domagała A, Kurpisz M. CD52 antigen--a review. Med Sci Monit. 2001 Mar-Apr;7(2):325-31. PubMed PMID: 11257744.
  5. Koyama K, Ito K, Hasegawa A. Role of male reproductive tract CD52 (mrt-CD52) in reproduction. Soc Reprod Fertil Suppl. 2007;63:103-10. PubMed PMID: 17566265.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Protein A Chromatography
Buffer System:
PBS, pH~7.4
Preservatives:
15 mM Sodium Azide
State:
Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody Clone HI186 reacts with CD52, a 21-28 kDa glycoprotein containing a large N-linked carbohydrate moiety. Mature CD52 molecule is actually much smaller (approx. 8-9 kDa).
Gene ID 1043

Accessory Products

Proteins and/or Positive Controls

Proteins for CD52 / CAMPATH-1 (1 products)

Catalog No. Species Pres. Purity   Source  

CD52 / CAMPATH-1

CD52 / CAMPATH-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.
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