AM06017FC-N CD59 antibody

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0.1 mg / €290.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD59 1F5

Product Description for CD59

Mouse anti Human CD59 1F5.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD59

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin
Presentation FITC
Reactivity Hu
Applications F
Clonality Monoclonal
Clone 1F5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P13987
Immunogen:
Mouse pre-B tumour cells (RAW112)
Donor: Lewis rat spleen
Fusion Partner: S 194/5. XXO. BU-1
GeneID:
966
Isotype control SM10F (for use in human samples)
Application Tissue Distribution by Flow Cytometry Analysis:
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 1.0μg/10e6 cells
Cell Source:
RBC 100%
Lymphocytes 80.2%
Background CD59 (also known as HRF20, protectin) is a 20 kDa glycoprotein attached to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. It blocks MAC formation in the complement pathway by interfering with C9 membrane insertion and polymerization, but it does not affect the generation of C3 and C5 activation products. CD59 is expressed on several cell types, including leukocytes, endothelial and epithelial cells, erythrocytes, and cells of the central nervous system. CD59 belongs to the Ly6 superfamily of proteins.
Concentration 0,1 mg/ml
General Readings
  1. Yu, J., et al. 1997. Mapping the active site of CD59. J. Exp. Med. 185 (4): 745-753.
  2. Farkas, I., et al. 2002. CD59 blocks not only the insertion of C9 into MAC but inhibits ion channel formation by homologous C5b-8 as well as C5b-9. J. of Physiol. 539 (2): 537-545.
  3. Zheng. H., et al. 1999. Targeting of functional antibody-CD59 fusion proteins to a cell surface. J. Clin. Invest. 103: 55-61.
  4. Okada, N., et al. 1989. Monoclonal antibodies capable of causing hemolysis of neuraminidase-treated human erythrocytes by homologous complement. J. of Immuol. 143 (7): 2262-2266.
  5. Yu, J., et al. 1997. The affected gene underlying the class K glycosylphosphatidylinositol (GPI) surface protein defect codes for the GPI transamidase. Proc. Natl. Acad. Sci. 94: 12580-12585.
  6. Chen, R., et al. 2000. Impaired growth and elevated Fas receptor expression in PIGA+ stem cells in primary paroxysmal nocturnal hemoglobinuria. J. Clin. Invest. 106: 689-696.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein isothiocyanate isomer 1
FITC
Buffer System:
PBS containing 0.02% sodium azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
State:
Liquid purified Ig fraction
Specificity
Specificity:
This antibody reacts to CD59.
Species:
Human.
Gene ID 966

Accessory Products

Proteins and/or Positive Controls

Proteins for CD59 (5 products)

Catalog No. Species Pres. Purity   Source  

CD59 (transcript variant 2)

CD59 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

CD59 (26-102, His-tag)

CD59 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  OriGene Technologies GmbH

CD59 (26-102, His-tag)

CD59 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  OriGene Technologies GmbH

CD59

CD59 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD59

CD59 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for CD59 (3 products)

Catalog No. Species Pres. Purity   Source  

CD59 293T Cell Transient Overexpression Lysate(Denatured)

CD59 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD59 293T Cell Transient Overexpression Lysate(Denatured)

CD59 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD59 Lysate

Western Blot: CD59 Lysate [NBL1-08958] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CD59
  Novus Biologicals Inc.
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