AM08314PU-N CD59 antibody

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50 µg / €380.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human, Mouse CD59 MEM-43/5

Product Description for CD59

Mouse anti Human, Mouse CD59 MEM-43/5.
Presentation: Purified
Product is tested for Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot, Flow Cytometry.

Properties for CD59

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin
Presentation Purified
Reactivity Hu, Ms
Applications F, IP, P, WB
Clonality Monoclonal
Clone MEM-43/5
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P13987
Immunogen:
Thymocytes and T lymphocytes.
GeneID:
966
Isotype control AM03110PU-N, SM12P
Application Flow Cytometry: 1 µg/ml.
Immunohistochemistry on Paraffin Sections: 5 µg/ml.
Immunoprecipitation.
Western Blot: 1 - 2 µg/ml.
Background CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. It is a potent inhibitor of the complement membrane attack complex action. It acts by binding to the C8 and/or C9 complements of the membrane attack complex, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is also involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
Concentration 1 mg/ml
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A Chromatography.
Buffer System:
PBS, pH 7.4 containing 15 mM Sodium Azide as preservative.
State:
Liquid purified Ig fraction.
Purified
Species Reactivity
Species reactivity (expected):
Mouse.
Species reactivity (tested):
Human.
Specificity
Specificity:
The antibody AM08314PU-N reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.
Gene ID 966

Accessory Products

Proteins and/or Positive Controls

Proteins for CD59 (5 products)

Catalog No. Species Pres. Purity   Source  

CD59 (transcript variant 2)

CD59 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

CD59 (26-102, His-tag)

CD59 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  OriGene Technologies GmbH

CD59 (26-102, His-tag)

CD59 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  OriGene Technologies GmbH

CD59

CD59 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD59

CD59 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for CD59 (3 products)

Catalog No. Species Pres. Purity   Source  

CD59 293T Cell Transient Overexpression Lysate(Denatured)

CD59 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD59 293T Cell Transient Overexpression Lysate(Denatured)

CD59 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD59 Lysate

Western Blot: CD59 Lysate [NBL1-08958] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CD59
  Novus Biologicals Inc.
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