AM26551FC-S CD59 antibody

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0.1 ml / €400.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human CD59 WK8

AM26551FC-S

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  • AM26551FC-S

Product Description for CD59

Mouse anti Human CD59 WK8.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD59

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin
Presentation FITC
Reactivity Hu
Applications F
Clonality Monoclonal
Clone WK8
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P13987
Immunogen:
Human myeloma cell line (RPMI8226)
GeneID:
966
Isotype control SM12F
Add. information

This product was originally produced by MBL International.

Application Flow cytometry: 10-20 μg/mL (final concentration).
For details see protocols below.
Background CD59, an 18-20 kDa GPI-anchored glycoprotein, is broadly distributed, present in most tissues, and on all circulating cells. CD59 has been also known as 20 kDa homologous restriction factor (HRF20), membrane attack complex inhibitory factor (MACIF), membrane inhibitor of reactive lysis (MIRL) and protectin. CD59 inhibits the cytolytic activity of complement by binding to C8 and C9 in the assembling of the cytolytic membrane attack complex (MAC), thereby interfering with membrane insertion and pore formation. CD59 and other membrane complement regulatory proteins (CD46 and CD55) are frequently overexpressed on cancer cells, possibly as a mechanism for cancer cells to overcome lysis by complement. CD59 also interacts with T cell-restricted glycoprotein CD2 and plays a role in T cell activation.
Concentration 0.5 mg/ml
Protocols

Flow cytometric analysis for whole blood cells
We usually use Falcon tubes or equivalents as reaction tubes for all steps described below.
1) Add 20 µL of the primary antibody at the concentration as suggest in the APPLICATIONS diluted with the washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN3] into each tube.
2) Add 100 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at room temperature (20~25oC).
3) Add 1 mL of washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
4) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (for analysis on BD instruments), using the procedure recommended in the respective package inserts.
5) Add 1 mL of H2O to each tube and incubate for 10 minutes at room temperature.
6) Centrifuge at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
7) Add 1 mL of washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
8) Resuspend the cells with 500 µ L of the washing buffer and analyze by a flow cytometer.

Flow cytometric analysis for floating cells
We usually use Fisher tubes or equivalents as reaction tubes for all steps described below.
1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN3].
2) Resuspend the cells with washing buffer (5 x 10e6 cells/mL).
3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25oC). Remove supernatant by careful aspiration.
4) Add 20 µL of human Fc receptor blocking reagent to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature.
5) Add 20 µL of the primary antibody at the concentration as suggest in the APPLICATIONS diluted in the washing buffer. Mix well and incubate for 30 minutes at room temperature.
6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.
(Positive controls for Flow cytometry; HPB-ALL, HL-60)

 

General Readings
  1. Davies, A., et al., J. Exp. Med. 170, 637-654 (1989).
  2. Okada, N., et al., Int. Immunol. 1, 205-208 (1989).
Storage Store at 2-8 °C.
Shelf life: one year from despatch.
Format
Label:
FITC
Purification:
Protein A agarose
Buffer System:
PBS containing 1% BSA and 0.09% NaN3
State:
Liquid Ig fraction
Specificity
Specificity:
This antibody reacts with human CD59 antigen.
Gene ID 966

Accessory Products

Proteins and/or Positive Controls

Proteins for CD59 (5 products)

Catalog No. Species Pres. Purity   Source  

CD59 (transcript variant 2)

CD59 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

CD59 (26-102, His-tag)

CD59 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  OriGene Technologies GmbH

CD59 (26-102, His-tag)

CD59 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  OriGene Technologies GmbH

CD59

CD59 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD59

CD59 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for CD59 (3 products)

Catalog No. Species Pres. Purity   Source  

CD59 293T Cell Transient Overexpression Lysate(Denatured)

CD59 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD59 293T Cell Transient Overexpression Lysate(Denatured)

CD59 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD59 Lysate

Western Blot: CD59 Lysate [NBL1-08958] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CD59
  Novus Biologicals Inc.
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