SM3033P CD59 antibody

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0.1 mg / €210.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD59 MEM-43/5

Product Description for CD59

Mouse anti Human CD59 MEM-43/5.
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for CD59

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin
Presentation Purified
Reactivity Hu
Applications F, IP, P, WB
Clonality Monoclonal
Clone MEM-43/5
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P13987
Immunogen:
Thymocytes and T lymphocytes
GeneID:
966
Isotype control AM03110PU-N, SM12P
Application Immunohistochemistry (paraffin sections)
Recommended dilution: 5 μg/ml.
Western Blotting: 1-2 µg/ml (non-reducing conditions).
Flow Cytometry: 1 µg/ml.
Positive control: blood.
Immunoprecipitation.
Background CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Concentration 1.0 mg/ml
General Readings 1. Meri S, Morgan BP, Davies A, Daniels RH, Olavesen MG, Waldmann H, Lachmann PJ: Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers. Immunology. 1990 Sep;71(1):1-9.
2. Rooney IA, Davies A, Griffiths D, Williams JD, Davies M, Meri S, Lachmann PJ, Morgan BP: The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells. Clin Exp Immunol. 1991 Feb;83(2):251-6.
3. Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE: CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation. J Immunol. 1994 Sep 15;153(6):2444-56.
4. Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP: CD59a is the primary regulator of membrane attack complex assembly in the mouse. J Immunol. 2004 Sep 15;173(6):3684-92.
5. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Bodian DL, Davis SJ, Morgan BP, Rushmere NK.: Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59. J Exp Med. 1997 Feb 3;185(3):507-16.
6. Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
7. Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1;272(31):19242-7.
Storage Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
Buffer System:
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
State:
Liquid Ig fraction
Purified
Specificity
Specificity:
The antibody reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues.
The antibody does not compete with most other CD59 antibodies.
Species:
Human, Mouse.
Gene ID 966
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for CD59 (5 products)

Catalog No. Species Pres. Purity   Source  

CD59 (transcript variant 2)

CD59 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

CD59 (26-102, His-tag)

CD59 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

CD59 (26-102, His-tag)

CD59 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

CD59

CD59 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD59

CD59 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for CD59 (3 products)

Catalog No. Species Pres. Purity   Source  

CD59 293T Cell Transient Overexpression Lysate(Denatured)

CD59 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD59 293T Cell Transient Overexpression Lysate(Denatured)

CD59 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD59 Lysate

Western Blot: CD59 Lysate [NBL1-08958] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CD59
  Novus Biologicals Inc.
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