AM12142PU-N CD66a/c/d/e antibody

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0.1 mg / €260.00
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Mouse anti Human CD66a/c/d/e CLB-gran/10

AM12142PU-N

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  • AM12142PU-N

Product Description for CD66a/c/d/e

Mouse anti Human CD66a/c/d/e CLB-gran/10.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.

Properties for CD66a/c/d/e

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CEACAM1, CEACAM3, CEACAM5, CEACAM6
Presentation Aff - Purified
Reactivity Hu
Applications F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone CLB-gran/10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human granulocytes
Application Flow cytometry: 2-8 μg/ml.
Immunoprecipitation.
Western blot.
Immunocytochemistry.
Immunohistochemistry on paraffin sections: 20 μg/ml. Positive tissue:human colon.
Background The CD66 molecules are 180-200 kDa glycoproteins of carcinoembryonic antigen family. They are present on all blood granulocytes and some tissue macrophages, but are absent from other hematopoietic cells. The expression of CD66 increases significantly on granulocytes upon their activation. CD66a (BGP1, CEACAM1) and CD66d (CGM1, CEACAM3), as well as CEACAM2 and 4, are transmembrane proteins, whereas CD66c (CEAL, NCA, CEACAM6) and CD66e (CEA, CEACAM5), as well as CEACAM7 and 8, are anchored to the plasma membrane by C-terminal glycosylphosphatidylinositol (GPI) lipid moiety.
Concentration 1.0 mg/ml
General Readings
  1. Singer BB, Scheffrahn I, Kammerer R, Suttorp N, Ergun S, Slevogt H. Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells. PLoS One. 2010 Jan 18;5(1):e8747. doi: 10.1371/journal.pone.0008747. PubMed PMID: 20090913. (Free PMC Article available, 6 images available)
  2. Tetteroo PA, Bos MJ, Visser FJ, von dem Borne AE. Neutrophil activation detected by monoclonal antibodies. J Immunol. 1986 May 1;136(9):3427-32. PubMed PMID: 2937846.
  3. Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  4. Nagel G, Grunert F, Kuijpers TW, Watt SM, Thompson J, Zimmermann W. Genomic organization, splice variants and expression of CGM1, a CD66-related member of the carcinoembryonic antigen gene family. Eur J Biochem. 1993 May 15;214(1):27-35. PubMed PMID: 8508798.
  5. Teixeira AM, Fawcett J, Simmons DL, Watt SM. The N-domain of the biliary glycoprotein (BGP) adhesion molecule mediates homotypic binding: domain interactions and epitope analysis of BGPc. Blood. 1994 Jul 1;84(1):211-9. PubMed PMID: 7517208.
Storage Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A affinity chromatography (> 95% pure by SDS-PAGE)
Buffer System:
Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
This antibody detects human CD66acde antigen, a granulocyte marker, especially after cell stimulation. The antibody does not cross-react with normal human peripheral B cells, T cells, monocytes and platelets. Weak reactivity has been observed with malignant cells of pacients with B cell-derived CLL. In immunohistochemistry the antibody reacts with some tissue macrophages and carcinoma-expressed CEA.

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