SM1550PS CD68 antibody

See related secondary antibodies

Search for all "CD68"

0.1 mg / €410.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Mouse CD68 FA-11


More Views

  • SM1550PS
  • SM1550PS
  • SM1550PS

Product Description for CD68

Rat anti Mouse CD68 FA-11.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for CD68

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Gp110, Macrophage marker, Macrosialin
Presentation Purified
Reactivity Ms
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone FA-11
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Purified Concanavalin A acceptor glycoprotein from P815 cell line.
Isotype control SM15P, SM15PX
Application Western Blot: Non-reducing conditions recommended.
Flow Cytometry:
Use 10 µl of 1/50-1/100 diluted antibody to label 10cells in 100 µl (Membrane permeabilization is required). 
Clone FA-11 can be used in Flow Cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident Mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Rat anti Mouse CD68 antibody, Clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues, See Masaki, T. et al. and Devey, L. et al. as examples.
-This product may require antigen retrieval using heat treatment prior to staining of
paraffin sections. Either sodium citrate buffer or Tris/EDTA buffer may be used for this
purpose. See Martin-Manso for details.
-Staining has also been achieved without antigen retrieval, See Lu et al. for details.
Background CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.
CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used in cancer studies to demonstrate the presence/localization of macrophages.
Concentration 1.0 mg/ml
Product Citations
Purchased from Acris:
  1. Kirby AC, Beattie L, Maroof A, van Rooijen N, Kaye PM. SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages. Am J Pathol. 2009 Sep;175(3):1107-15. doi: 10.2353/ajpath.2009.090258. Epub 2009 Jul 30. PubMed PMID: 19644016. (Free PMC Article available, 5 images available)
  2. Zimmermann O, Homann JM, Bangert A, Müller AM, Hristov G, Goeser S, et al. Successful use of mRNA-nucleofection for overexpression of interleukin-10 in murine monocytes/macrophages for anti-inflammatory therapy in a murine model of autoimmune myocarditis. J Am Heart Assoc. 2012 Dec;1(6):e003293. doi: 10.1161/JAHA.112.003293. Epub 2012 Dec 19. PubMed PMID: 23316321. (Free PMC Article available, 8 images available)
  3. Jehle J, Hoyer FF, Schöne B, Pfeifer P, Schild K, Jenniches I, et al. Myeloid-Specific Deletion of Diacylglycerol Lipase α Inhibits Atherogenesis in ApoE-Deficient Mice. PLoS One. 2016 Jan 5;11(1):e0146267. doi: 10.1371/journal.pone.0146267. eCollection 2016. PubMed PMID: 26731274. (Free PMC Article available, 5 images available)
  4. Asdonk, T;Steinmetz, M;Krogmann, A;Ströcker, C;Lahrmann, C;Motz, I;Paul-Krahe, K;Flender, A;Schmitz, T;Barchet, W;Hartmann, G;Nickenig, G;Zimmer, S. MDA-5 activation by cytoplasmic double-stranded RNA impairs endothelial function and aggravates atherosclerosisJ. Cell. Mol. Med. 2016. PubMed PMD: 27130701.
General Readings 1. Ramprasad MP, Terpstra V, Kondratenko N, Quehenberger O, Steinberg D. Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein. Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14833-8. PubMed PMID: 8962141.
2. Rabinowitz SS, Gordon S. Macrosialin, a macrophage-restricted membrane sialoprotein differentially glycosylated in response to inflammatory stimuli. J Exp Med. 1991 Oct 1;174(4):827-36. PubMed PMID: 1919437.
3. da Silva RP, Gordon S. Phagocytosis stimulates alternative glycosylation of macrosialin (mouse CD68), a macrophage-specific endosomal protein. Biochem J. 1999 Mar 15;338 ( Pt 3):687-94. PubMed PMID: 10051440.
4. Schleicher U, Hesse A, Bogdan C. Minute numbers of contaminant CD8+ T cells or CD11b+CD11c+ NK cells are the source of IFN-gamma in IL-12/IL-18-stimulated mouse macrophage populations. Blood. 2005 Feb 1;105(3):1319-28. Epub 2004 Sep 21. PubMed PMID: 15383459.
5. von Lukowicz T, Hassa PO, Lohmann C, Borén J, Braunersreuther V, Mach F, et al. PARP1 is required for adhesion molecule expression in atherogenesis. Cardiovasc Res. 2008 Apr 1;78(1):158-66. Epub 2007 Dec 18. PubMed PMID: 18093987.
6. Kassim SH, Li H, Vandenberghe LH, Hinderer C, Bell P, Marchadier D, et al. Gene therapy in a humanized mouse model of familial hypercholesterolemia leads to marked regression of atherosclerosis. PLoS One. 2010 Oct 19;5(10):e13424. doi: 10.1371/journal.pone.0013424. PubMed PMID: 20976059.
7. Rahaman SO, Swat W, Febbraio M, Silverstein RL. Vav family Rho guanine nucleotide exchange factors regulate CD36-mediated macrophage foam cell formation. J Biol Chem. 2011 Mar 4;286(9):7010-7. doi: 10.1074/jbc.M110.192450. Epub 2011 Jan 5. PubMed PMID: 21209086.
8. Frossard JL, Lenglet S, Montecucco F, Steffens S, Galan K, Pelli G, et al. Role of CCL-2, CCR-2 and CCR-4 in cerulein-induced acute pancreatitis and pancreatitis-associated lung injury. J Clin Pathol. 2011 May;64(5):387-93. doi: 10.1136/jcp.2010.088500. Epub 2011 Feb 23. PubMed PMID: 21345872.
9. West EL, Pearson RA, Barker SE, Luhmann UF, Maclaren RE, Barber AC, et al. Long-term survival of photoreceptors transplanted into the adult murine neural retina requires immune modulation. Stem Cells. 2010 Nov;28(11):1997-2007. doi: 10.1002/stem.520. PubMed PMID: 20857496.
10. Lopez ME, Klein AD, Dimbil UJ, Scott MP. Anatomically defined neuron-based rescue of neurodegenerative Niemann-Pick type C disorder. J Neurosci. 2011 Mar 23;31(12):4367-78. doi: 10.1523/JNEUROSCI.5981-10.2011. PubMed PMID: 21430138.
11. Jayagopal A, Su YR, Blakemore JL, Linton MF, Fazio S, Haselton FR. Quantum dot mediated imaging of atherosclerosis. Nanotechnology. 2009 Apr 22;20(16):165102. doi: 10.1088/0957-4484/20/16/165102. Epub 2009 Mar 31. PubMed PMID: 19420562.
12. Leung VW, Yun S, Botto M, Mason JC, Malik TH, Song W, et al. Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice. Am J Pathol. 2009 Oct;175(4):1757-67. doi: 10.2353/ajpath.2009.090183. Epub 2009 Sep 3. PubMed PMID: 19729477.
13. Devey L, Ferenbach D, Mohr E, Sangster K, Bellamy CO, Hughes J, et al. Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism. Mol Ther. 2009 Jan;17(1):65-72. doi: 10.1038/mt.2008.237. Epub 2008 Nov 11. PubMed PMID: 19002167.
14. Lu W, Huang Q, Ku G, Wen X, Zhou M, Guzatov D, et al. Photoacoustic imaging of living mouse brain vasculature using hollow gold nanospheres. Biomaterials. 2010 Mar;31(9):2617-26. doi: 10.1016/j.biomaterials.2009.12.007. Epub 2009 Dec 24. PubMed PMID: 20036000.
15. de Beer MC, Zhao Z, Webb NR, van der Westhuyzen DR, de Villiers WJ. Lack of a direct role for macrosialin in oxidized LDL metabolism. J Lipid Res. 2003 Apr;44(4):674-85. Epub 2003 Jan 16. PubMed PMID: 12562841.
16. Song L, Lee C, Schindler C. Deletion of the murine scavenger receptor CD68. J Lipid Res. 2011 Aug;52(8):1542-50. doi: 10.1194/jlr.M015412. Epub 2011 May 13. PubMed PMID: 21572087.
17. Daldrup-Link HE, Golovko D, Ruffell B, Denardo DG, Castaneda R, Ansari C, et al. MRI of tumor-associated macrophages with clinically applicable iron oxide nanoparticles. Clin Cancer Res. 2011 Sep 1;17(17):5695-704. doi: 10.1158/1078-0432.CCR-10-3420. Epub 2011 Jul 26. PubMed PMID: 21791632.
18. Macauley SL, Pekny M, Sands MS. The role of attenuated astrocyte activation in infantile neuronal ceroid lipofuscinosis. J Neurosci. 2011 Oct 26;31(43):15575-85. doi: 10.1523/JNEUROSCI.3579-11.2011. PubMed PMID: 22031903.
19. Martin-Manso G, Galli S, Ridnour LA, Tsokos M, Wink DA, Roberts DD. Thrombospondin 1 promotes tumor macrophage recruitment and enhances tumor cell cytotoxicity of differentiated U937 cells. Cancer Res. 2008 Sep 1;68(17):7090-9. doi: 10.1158/0008-5472.CAN-08-0643. PubMed PMID: 18757424.
20. Lazarini F, Gabellec MM, Torquet N, Lledo PM. Early activation of microglia triggers long-lasting impairment of adult neurogenesis in the olfactory bulb. J Neurosci. 2012 Mar 14;32(11):3652-64. doi: 10.1523/JNEUROSCI.6394-11.2012. PubMed PMID: 22423088.
21. Hemmi H, Idoyaga J, Suda K, Suda N, Kennedy K, Noda M, et al. A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells. J Immunol. 2009 Feb 1;182(3):1278-86. PubMed PMID: 19155473.
22. Akbarshahi H, Menzel M, Posaric Bauden M, Rosendahl A, Andersson R. Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury. PLoS One. 2012;7(10):e42654. doi: 10.1371/journal.pone.0042654. Epub 2012 Oct 22. PubMed PMID: 23110041.
23. Smith MJ, Koch GL. Differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes. J Cell Sci. 1987 Feb;87 ( Pt 1):113-9. PubMed PMID: 3312248.
24. Masaki T, Chow F, Nikolic-Paterson DJ, Atkins RC, Tesch GH. Heterogeneity of antigen expression explains controversy over glomerular macrophage accumulation in mouse glomerulonephritis. Nephrol Dial Transplant. 2003 Jan;18(1):178-81. PubMed PMID: 12480978.
25. Dormishian M, Turkeri G, Urayama K, Nguyen TL, Boulberdaa M, Messaddeq N, et al. Prokineticin receptor-1 is a new regulator of endothelial insulin uptake and capillary formation to control insulin sensitivity and cardiovascular and kidney functions. J Am Heart Assoc. 2013 Oct 23;2(5):e000411. doi: 10.1161/JAHA.113.000411. PubMed PMID: 24152983.
26. von Bargen K, Gagnaire A, Arce-Gorvel V, de Bovis B, Baudimont F, Chasson L, et al. Cervical Lymph Nodes as a Selective Niche for Brucella during Oral Infections. PLoS One. 2015 Apr 28;10(4):e0121790. doi: 10.1371/journal.pone.0121790. eCollection 2014. PubMed PMID: 25919005.
27. Hamour S, Gan PY, Pepper R, Florez Barros F, Wang HH, O'Sullivan K, et al. Local IL-17 Production Exerts a Protective Role in Murine Experimental Glomerulonephritis. PLoS One. 2015 Aug 28;10(8):e0136238. doi: 10.1371/journal.pone.0136238. eCollection 2015. PubMed PMID: 26317864.
28. Wang L, Kang S, Zou D, Zhan L, Li Z, Zhu W, et al. Bone Fracture Pre-Ischemic Stroke Exacerbates Ischemic Cerebral Injury in Mice. PLoS One. 2016 Apr 18;11(4):e0153835. doi: 10.1371/journal.pone.0153835. eCollection 2016. PubMed PMID: 27089041.
29. Nguyen, T.V. et al. (2016) Multiplex immunoassay characterization and species comparison of inflammation in acute and non-acute ischemic infarcts in human and mouse brain tissue.
Acta Neuropathol Commun. 4 (1): 100.
30. Pena-Philippides, J.C. et al. (2016) In vivo inhibition of miR-155 significantly alters Page 3 of 5
post-stroke inflammatory response. J Neuroinflammation. 13 (1): 287.
31. Paiva, A. A. et al. (2017) Apolipoprotein CIII Overexpression-Induced Hypertriglyceridemia Increases Nonalcoholic Fatty Liver Disease in Association with Inflammation and Cell Death.
Oxidative Med Cellular Longev. 2017: 1-18.
32. Giraldo, J.A. et al. (2016) The impact of cell surface PEGylation and short-course immunotherapy on islet graft survival in an allogeneic murine model. Acta Biomater. pii: S1742-7061(16)30656-0. [Epub ahead of print]
33. Masuda, T. et al. (2017) Growth Factor Midkine Promotes Nuclear Factor of Activated T Cells-Regulated T-Cell-Activation and Th1 Cell Differentiation in Lupus Nephritis. Am J Pathol.
Feb 6. pii: S0002-9440(17)30029-9. [Epub ahead of print]
34. Garofalo, S. et al. (2017) The glycoside oleandrin reduces glioma growth with direct and indirect effects on tumor cells. J Neurosci. Mar 14. pii: 2296-16. [Epub ahead of print]
35. Maeda, K. et al. (2017) Inhibition of H3K9 methyltransferase G9a ameliorates methylglyoxal-induced peritoneal fibrosis. PLoS One. 12 (3): e0173706.
36. Nishikawa, K. et al. (2015) Resveratrol increases CD68⁺ Kupffer cells colocalized with adipose differentiation-related protein and ameliorates high-fat-diet-induced fatty liver in mice.
Mol Nutr Food Res. 59 (6): 1155-70.

Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G
Buffer System:
0.09% Sodium Azide
Liquid purfied IgG fraction from Tissue Culture Supernatant
Clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.
Gene ID 12514
FocusOn and Reviews

Accessory Products

  • LinkedIn