AM32811PU-S CD74 antibody

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0.1 mg / €320.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human, Mouse, Baboon CD74 LN2

Product Description for CD74

Mouse anti Human, Mouse, Baboon CD74 LN2.
Presentation: Purified
Product is tested for Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Frozen Sections, Flow Cytometry, Western blot / Immunoblot, Paraffin Sections.

Properties for CD74

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, p33
Presentation Purified
Reactivity Bab, Hu, Ms
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone LN2
Host Mouse
Isotype IgG1
Molecular weight 33-41 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P04233
Immunogen:
SU-DHL-4 lymphoma cells.
GeneID:
972
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: For coating, Use Antibody without BSA. 
Western Blot:
 0.5-1 µg/ml.
Flow Cytometry: 
0.5-1 µg/106 cells.
Immunofluorescence:
 1-2 µg/ml. 
Immunoprecipitation: 1-2 µg/500 µg protein lysate.
Immunohistochemistry on Frozen and Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: Daudi Cells or Raji Cells. Tonsil or lymph node.
Background CD74, a Type-II transmembrane protein, is a unique member of RIP-processed protein family. It consists of an extracytoplasmic domain, a transmembrane segment and an intracytoplasmic domain. It is expressed in B-cells, monocytes, macrophages and other Class II MHC expressing cells. CD74 associates with alpha and beta chains of HLA-DR (MHC class II) and directs intracellular sorting of MHC class II molecules. It has a prominent role in antigen presentation and as an accessory signaling molecule. Cell-surface CD74 binds to MIF and its expression is required for MIF-mediated ERK1/2-phosphorylation, PGE2 production and cell proliferation. CD74 regulates B-cell differentiation and acts as a survival receptor through activation of NF-kB p65/RelA homodimer. Pathological role of CD74 has been identified in different cancers including Multiple Myeloma.
Concentration 0.2 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Zheng YX, Yang M, Rong TT, Yuan XL, Ma YH, Wang ZH, et al. CD74 and macrophage migration inhibitory factor as therapeutic targets in gastric cancer. World J Gastroenterol. 2012 May 14;18(18):2253-61. doi: 10.3748/wjg.v18.i18.2253. PubMed PMID: 22611320. (Free PMC Article available, 4 images available)
General Readings
  1. Leng et.al JEM 197 (11); 1467-1476 (June 2003).
  2. Becker-Herman et.al MBC 16 (11) 5061-5069 (November 2005).
  3. Diana et.al Blood 107 (12) 4807-4816 (15 June 2006).
  4. Ioachim HL, Pambuccian S, Giancotti F, Dorsett B. Reactivity of lung tumors with lung-derived and non-lung-derived monoclonal antibodies. Int J Cancer Suppl. 1994;8:132-3. PubMed PMID: 7515026.
  5. Epstein AL, Marder RJ, Winter JN, Fox RI. Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984 Aug;133(2):1028-36. PubMed PMID: 6376628.
  6. Marder RJ, Variakojis D, Silver J, Epstein AL. Immunohistochemical analysis of human lymphomas with monoclonal antibodies to B cell and Ia antigens reactive in paraffin sections. Lab Invest. 1985 May;52(5):497-504. PubMed PMID: 3887032.
  7. Kumar S, Kumar D, Gourley WK, Alperin JB. Sporotrichosis as a presenting manifestation of hairy cell leukemia. Am J Hematol. 1994 Jun;46(2):134-7. PubMed PMID: 8172180.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10 mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Species Reactivity
Species reactivity (tested):
Human, Baboon and Mouse.
Specificity
Specificity:
This monoclonal LN2 antibody can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells.  
It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV).
Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
Cellular Localization: Cell surface and paranuclear globular.
Negative Species: Rat.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD74 (9 products)

Catalog No. Species Pres. Purity   Source  

CD74 (transcript variant 1)

CD74 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD74 (73-232, His-tag)

CD74 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  OriGene Technologies GmbH

CD74 (73-232, His-tag)

CD74 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

CD74 (73-232, His-tag)

CD74 Human Purified > 85 % by SDS - PAGE.
0.25 mg / €940.00
  OriGene Technologies GmbH

CD74 (73-232, His-tag)

CD74 Human Purified > 85 % by SDS - PAGE.
50 µg / €300.00
  OriGene Technologies GmbH

CD74

CD74 Human
  Abnova Taiwan Corp.

CD74

CD74 Human in vitro transl.
  Abnova Taiwan Corp.

CD74

CD74 Human in vitro transl.
  Abnova Taiwan Corp.

CD74

CD74 Human in vitro transl.
  Abnova Taiwan Corp.

Positive controls for CD74 (5 products)

Catalog No. Species Pres. Purity   Source  

CD74 Lysate

Western Blot: CD74 Lysate [NBL1-08968] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CD74
  Novus Biologicals Inc.

CD74 overexpression lysate

CD74 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CD74 overexpression lysate

CD74 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CD74 overexpression lysate

CD74 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CD74 overexpression lysate

CD74 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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