TA309313 CD74 antibody

Mouse Monoclonal anti-CD74 Antibody

See related secondary antibodies

Search for all "CD74"

0.1 mg / €342.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD74 PIN.1

Product Description for CD74

Mouse anti Human CD74 PIN.1.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for CD74

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, p33
Presentation Purified
Reactivity Hu
Applications C, ICC/IF, IP, WB
Clonality Monoclonal
Clone PIN.1
Host Mouse
Isotype IgG1
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P04233
Immunogen:
Human CD74 invariant chain synthetic peptide conjugated to KLH
GeneID:
972
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western blot (1 µg/ml was sufficient for detection using colorimetric western blot analysis).
Immunoprecipitation(5).
Immunoflourescence(6).
Flow cytometry.
FACS (6).
Background CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
Concentration 1.0 mg/ml
General Readings
  1. Becker-Hermann, S., Arie, G., Medvedovsky H, Kerem A, and Shachar I. (2005) Mol Bio Cell. 16(11):5061-9.
  2. Barrera CA., et al (2005) J Histochem Cytochem 53 (12): 1481-9.
  3. Starlets D., et al. (2006) Blood. 107 (12): 4807-4816.
  4. Burton JD., et al. (2004). Clin Cancer Res. 10(19): 6606-11.
  5. Denzin L.K., Hammond, C. and Cresswell, P. (1996) J. Exp. Med. 184: 2153-2165.
  6. Denzin L.K., Robbins N.F., Carboy-Newcomb C. and Cresswell P. (1994) Immunity 1: 595-606.
Storage Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS, pH 7.2, 0.09 % azide and 50 % glycerol
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
Detects ~33-35 kDa protein doublet, corresponding to the molecular mass of the p33 and p35 forms of human CD74 on SDS-PAGE immunoblots.
Species:
Human.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD74 (9 products)

Catalog No. Species Pres. Purity   Source  

CD74 (transcript variant 1)

CD74 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD74 (73-232, His-tag)

CD74 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  OriGene Technologies GmbH

CD74 (73-232, His-tag)

CD74 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

CD74 (73-232, His-tag)

CD74 Human Purified > 85 % by SDS - PAGE.
0.25 mg / €940.00
  OriGene Technologies GmbH

CD74 (73-232, His-tag)

CD74 Human Purified > 85 % by SDS - PAGE.
50 µg / €300.00
  OriGene Technologies GmbH

CD74

CD74 Human
  Abnova Taiwan Corp.

CD74

CD74 Human in vitro transl.
  Abnova Taiwan Corp.

CD74

CD74 Human in vitro transl.
  Abnova Taiwan Corp.

CD74

CD74 Human in vitro transl.
  Abnova Taiwan Corp.

Positive controls for CD74 (5 products)

Catalog No. Species Pres. Purity   Source  

CD74 Lysate

Western Blot: CD74 Lysate [NBL1-08968] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CD74
  Novus Biologicals Inc.

CD74 overexpression lysate

CD74 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CD74 overexpression lysate

CD74 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CD74 overexpression lysate

CD74 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CD74 overexpression lysate

CD74 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
  • LinkedIn