DM117S CD8 antibody

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Mouse anti Human CD8 144B


Product Description for CD8

Mouse anti Human CD8 144B.
Presentation: Supernatant
Product is tested for Frozen Sections, Paraffin Sections.

Properties for CD8

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms CD8, MAL, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein CD8 beta chain, T-lymphocyte differentiation antigen T8/Leu-2
Presentation Supernatant
Reactivity Hu
Applications C, P
Clonality Monoclonal
Clone 144B
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Synthetic peptide sequence of cytoplasmic domain of the CD8 molecule.
Application This antibody can be used on formalin-fixed, paraffin embedded tissue sections, as well as on frozen tissue sections. For a three-step streptavidin-biotin complex detection system a dilution of 1:25 is recommended as a guideline. The incubation periods for 2 hours to overnight at room temperature provide uniform and reproducible results. To enhance staining, high temperature-based antigenic unmasking technique (see instructions below) is highly recommended when using paraffin-embedded, formalin-fixed sections. Recommended positive control: Human Tonsil, Spleen. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.19 mg/ml
General Readings
  1. Itzkowitz SH, et al. Cancer 66: 1960, 1990.
  2. Itzkowitz SH, et al. Cancer 49: 197, 1989.
  3. Kjeldsen T, et al. Cancer Res. 48: 2214, 1988
  4. Teresawa K., et al. Can
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
This antibody is specific against human CD8 antigen, a 32 kD transmembrane protein present in the cytotoxic/suppressor T lymphocyte subset of T8 cells.

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