SM3038PX CD8 antibody

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Mouse anti Human CD8 MEM-87


Product Description for CD8

Mouse anti Human CD8 MEM-87.
Presentation: Purified
Product is tested for Immunoprecipitation, Flow Cytometry.

Properties for CD8

Product Category Primary Antibodies
Quantity 1 mg
Synonyms CD8, MAL, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein CD8 beta chain, T-lymphocyte differentiation antigen T8/Leu-2
Presentation Purified
Reactivity Hu
Applications F, IP
Clonality Monoclonal
Clone MEM-87
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Leucocytes of a patient suffering from LGL Type Leukaemia
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunoprecipitation (Excellent for immunoisolation of CD8 + T cells).
Flow Cytometry: 2 μg/ml.
Background The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as ab heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as aa homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8b level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 a-b but not a-a dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
CD8 is a disulfide-linked dimer and exists as a CD8a homodimer or CD8a/b heterodimer (each monomer approx. 32-34 kDa).
Concentration 1.0 mg/ml
General Readings Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB: Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding. J Immunol. 2006 Sep 15;177(6):3930-8.
Pang DJ, Hayday AC, Bijlmakers MJ.: CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers. J Biol Chem. 2007 May 4;282(18):13884-94.
van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8-driven modulation of T cell antigen receptor specificity. J Theor Biol. 2007 Nov 21;249(2):395-408.
Storage Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
Buffer System:
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Liquid Ig fraction
The antibody recognizes CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.

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