NB200-581 Cdc6 antibody

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Mouse anti Human Cdc6 DCS-180


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  • NB200-581

Product Description for Cdc6

Mouse anti Human Cdc6 DCS-180.
Presentation: Purified
Product is tested for Frozen Sections, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for Cdc6

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms cdc 6, cdc6
Presentation Purified
Reactivity Hu
Applications C, IP, P, WB
Clonality Monoclonal
Clone DCS-180
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Recombinant full length protein (Human).
Isotype control AM03095PU-N, SM10P (for use in human samples)
Add. information Research Areas: Stem Cell Markers
Application immunoprecipitation, Western Blot, Western Blotting: use at a concentration of 1-2ug / ml for 2hrs at RT. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. By Western blot, this antibody detects a band of 62 kDa, which corresponds to the predicted molecular weight of Cdc 6., immunoprecipitation 2ug/mg, Western Blot 1-2ug / ml
Background The replication licensing system acts to ensure that no section of the genome is replicated more than once in a single cell cycle. The origin recognition complex (ORC) and CDC6/CDC18 are needed on chromatin before the licensing reaction can take place. During the transition from a growth-arrested to a proliferative state, transcription of mammalian CDC6 is regulated by the cell cycle transcription factor (E2F protein). Immunoblots show that minichromosome maintenance (MCM) proteins are not associated with chromatin. Competence of G1 phase nuclei to replicate in vitro coincides with maximum CDC6 accumulation and MCM protein binding to chromatin in vivo. Antibodies against CDC6 and MCM5 stain abnormal (neoplastically transformed) cells in cervical smears and sections with remarkably high specificity and sensitivity.
General Readings General / background references:Petersen BO et al. Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization. EMBO J 18:396-410 (1999). PubMed PMID: 9889196
Storage Store at 4C. Do not freeze.
Protein G purified
Buffer System:
0.09% Sodium Azide
Protein G purified
Cdc 6 (DCS-180)

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