DM3106 CDK5 antibody

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Mouse anti Human, Mouse, Rat CDK5 DC17


Product Description for CDK5

Mouse anti Human, Mouse, Rat CDK5 DC17.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for CDK5

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Cell division protein kinase 5, Cyclin-dependent kinase 5, PSSALRE
Presentation Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone DC17
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Recombinant cdk5 protein
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Add. information Mol. Wt. of Antigen: 31kD
Application Immunofluorescence.
Immunoprecipitation (Native and denatured; 2µg/mg of protein lysate; Use Protein G).
Western Blotting (1-2µg/ml for 2hrs at RT).
Immunohistology (1-2µg/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min).
Recommended positive control: HT29 or MAD109 Cells, Brain.
Concentration 0.2 mg/ml
General Readings
  1. Meyerson M, et. al. (1992) EMBO J, 11:2909-2917.
  2. Nurse P and Bisset Y. (1981) Nature, 292:558-560.
  3. Matsushime H, et. al. (1991) Cell, 65:701-713.
  4. Xiong Y, et al. (1992) Cell, 71:505-514.
Storage Store the antibody at 2-8°C. Do not freeze! Shelf life: One year from despatch.
Protein G affinity purified immunoglobulin fraction
Buffer System:
10 mM PBS with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer
Cdk5 is expressed in both cycling and noncycling cells and is present in numerous mammalian tissues. Neuronal and muscle cells contain the highest amount of this protein. The p35 protein, which is expressed solely in the brain, activates Cdk5. Cellular Localization: cytoplasmic, nuclear.
Human, Mouse, and Rat. Does not react with C.elegans.

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