DM1231 CEACAM20 antibody

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0.1 mg / €330.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human CEACAM20 HT-12D8

DM1231

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Product Description for CEACAM20

Mouse anti Human CEACAM20 HT-12D8.
Presentation: Purified
Product is tested for ELISA (capture), Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for CEACAM20

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Carcinoembryonic antigen-related cell adhesion molecule 20
Presentation Purified
Reactivity Hu
Applications E, E(capture), F, ICC/IF
Clonality Monoclonal
Clone HT-12D8
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q6UY09
Immunogen:
Genetic immunisation with cDNA encoding Human CEACAM20.
GeneID:
125931
Remarks:
Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Add. information SDS-PAGE analysis: The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Figure.4).
Application Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
ELISA (capture): With clone HD-6G4-A5 as detection antibody.
Flow cytometry: 1.2 µg/10e6 cells.
Immunofluorescence: 1 µg/10e6 cells.
Background CEA-related cell adhesion molecule 20 (CEACAM20) belongs to the carcinoembryonic antigen (CEA) gene family (1). It encodes a putative glycoprotein which is membrane-bound via a transmembrane domain. The CEACAM20 protein contains a single N domain followed by 4 immunoglobulin-like A (A1, A2) and B (B1, B2) domains. Expression of CEACAM20 can be found in tissues of prostate, testis, duodenum and small intestine with highest expression in prostate. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. High expression of CEACAM20 in tissue of prostate carcinoma and in prostate carcinoma cell lines suggest that CEACAM20 can be used as a tumor marker.
Concentration 2.0 mg/ml
General Readings
  1. Zimmermann W (2002). Carcinoembryonic antigen. In Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York, USA, pp. 459-462.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G.
Buffer System:
Phosphate buffered saline, pH 7.2
State:
Liquid purified Ig fraction.
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
Recognizes CEACAM20
Gene ID 125931

Accessory Products

Proteins and/or Positive Controls

Proteins for CEACAM20 (1 products)

Catalog No. Species Pres. Purity   Source  

Carcinoembryonic antigen-related cell adhesion molecule 20 (CEACAM20), transcript variant 5L (transcript variant 5L)

Carcinoembryonic antigen-related cell adhesion molecule 20 (CEACAM20), transcript variant 5L Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.
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