discontinued

NB110-58737 cfp10 / esxB antibody

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Rabbit anti Bacteria cfp10 / esxB

Product Description for cfp10 / esxB

Rabbit anti Bacteria cfp10 / esxB.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for cfp10 / esxB

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms 10 kDa culture filtrate antigen cfp10, ESAT-6-like protein esxB, MT3988, MTV027.09, Rv3874, Secreted antigenic protein MTSA-10, lhp, mtsA10
Presentation Purified
Reactivity Bac
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P0A566
Immunogen:
CFP10 (Rv3874) of Mycobacterium tuberculosis
GeneID:
886194
Application Western Blot, Western Blot use reducing conditions., Western Blot 0.2-1ug/ml,
Background CFP10 (10 kDa culture filtrate protein) is a low molecular weight protein isolated from Mycobacterium tuberculosis culture filtrates. CFP10 and the 6 kDa early secretory antigen of T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG). CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, that appear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunization of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Concentration 1 mg/ml
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
protein A purified
Buffer System:
No Preservative
Purity:
protein A purified
State:
Purified
Specificity
Specificity:
This polyclonal antibody reacts with CFP10 (10 kDa culture filtrate antigen ESXB; LHP), a 12,3 kDa antigen encoded by gene Rv3874 of Mycobacterium tuberculosis. The antibody recognizes CFP10 of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis; it reacts also with recombinant antigen produced in Escherichia coli.
Species:
Bacteria
Gene ID 886194

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