SM2177 Chimaeric Human IgE anti Nitrophenyl-hapten (NP) antibody

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2 ml / €640.00
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Human anti Chimaeric Human IgE anti Nitrophenyl-hapten (NP) JW8/1


Product Description for Chimaeric Human IgE anti Nitrophenyl-hapten (NP)

Human anti Chimaeric Human IgE anti Nitrophenyl-hapten (NP) JW8/1.
Presentation: Supernatant
Product is tested for Enzyme Immunoassay.

Properties for Chimaeric Human IgE anti Nitrophenyl-hapten (NP)

Product Category Primary Antibodies
Quantity 2 ml
Presentation Supernatant
Applications E
Clonality Monoclonal
Clone JW8/1
Host Human
Isotype IgE
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Hapten, 4-hydroxy-3-nitrophenylacetyl (NP)
Plasmids containing chimeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line.
Application Suitable for ELISA (1/20-1/100).
Concentration 0.02-0.05 mg/ml
General Readings
  1. Neuberger MS, Williams GT, Fox RO. Recombinant antibodies possessing novel effector functions. Nature. 1984 Dec 13-19;312(5995):604-8. PubMed PMID: 6095112.
  2. Neuberger, M.S., (1985). Making Novel Antibodies by expressing transfected immunoglobulin genes. TIBS 347-349.
  3. Neuberger MS, Williams GT, Mitchell EB, Jouhal SS, Flanagan JG, Rabbitts TH. A hapten-specific chimaeric IgE antibody with human physiological effector function. Nature. 1985 Mar 21-27;314(6008):268-70. PubMed PMID: 2580239.
  4. Neuberger, M.S., Williams, G.T. (1986). Construction of novel antibodies by use of DNA transfection: design of plasmid vectors. Phil. Trans. R. Soc. Lond. A317: 425-432.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
Contains 0.2M Tris/HCl pH7.4 and 5-10% Foetal Calf Serum in PBS buffer pH 7.2 with 0.09% Sodium Azide as preservative.
Liquid Tissue Culture Supernatant.
The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures.
The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimaeric human IgE antibody specific for NP has been produced.

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