SM2178P Chimaeric Human IgG2 anti Nitrophenyl-hapten (NP) antibody

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0.5 mg / €560.00
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Human anti Human Chimaeric Human IgG2 anti Nitrophenyl-hapten (NP) JW183


Product Description for Chimaeric Human IgG2 anti Nitrophenyl-hapten (NP)

Human anti Human Chimaeric Human IgG2 anti Nitrophenyl-hapten (NP) JW183.
Presentation: Purified
Product is tested for Enzyme Immunoassay.

Properties for Chimaeric Human IgG2 anti Nitrophenyl-hapten (NP)

Product Category Primary Antibodies
Quantity 0.5 mg
Presentation Purified
Reactivity Hu
Applications E
Clonality Monoclonal
Clone JW183
Host Human
Isotype IgG2
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application ELISA.
Concentration 1.0 mg/ml
General Readings
  1. Neuberger MS, Williams GT, Fox RO. Recombinant antibodies possessing novel effector functions. Nature. 1984 Dec 13-19;312(5995):604-8. PubMed PMID: 6095112.
  2. Neuberger MS, Williams GT, Mitchell EB, Jouhal SS, Flanagan JG, Rabbitts TH. A hapten-specific chimaeric IgE antibody with human physiological effector function. Nature. 1985 Mar 21-27;314(6008):268-70. PubMed PMID: 2580239.
  3. Neuberger, M.S. et al.: Construction of novel antibodies by use of DNA transfection: design of plasmid vectors. Phil. Trans. R. Soc. Lond. A317: 425-432 (1986).
  4. Wines BD, Gavin A, Powell MS, Steinitz M, Buchanan RR, Mark Hogarth P. Soluble FcgammaRIIa inhibits rheumatoid factor binding to immune complexes. Immunology. 2003 Jun;109(2):246-54. PubMed PMID: 12757620. (Free PMC Article available, 8 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography
Buffer System:
0.09% Sodium azide
Liquid purified IgG fraction from tissue culture supernatant
IgG2 Anti NP (Chimaeric).
The Immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a Mouse hybridoma to Human constant region genes by in vitro DNA recombination procedures. The resulting Chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. The J558L cell-line itself secretes a lambda light chain but no heavy chain. Thus a chimaeric Human IgG2 antibody specific for NP has been produced.

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