SM2185 Chimaeric human IgM anti Nitrophenyl-hapten (NP) antibody

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2 ml / €530.00
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Human anti Chimaeric human IgM anti Nitrophenyl-hapten (NP) THM


Product Description for Chimaeric human IgM anti Nitrophenyl-hapten (NP)

Human anti Chimaeric human IgM anti Nitrophenyl-hapten (NP) THM.
Presentation: Supernatant
Product is tested for Enzyme Immunoassay.

Properties for Chimaeric human IgM anti Nitrophenyl-hapten (NP)

Product Category Primary Antibodies
Quantity 2 ml
Presentation Supernatant
Applications E
Clonality Monoclonal
Clone THM
Host Human
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Hapten, 4-hydroxy-3-nitrophenylacetyl (NP).
Plasmids containing chimaeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line.
Application ELISA.
General Readings
  1. Neuberger MS, Williams GT, Fox RO. Recombinant antibodies possessing novel effector functions. Nature. 1984 Dec 13-19;312(5995):604-8. PubMed PMID: 6095112.
  2. Neuberger, M.S. (1985) Making Novel Antibodies by expressing transfected immunoglobulin genes. TIBS 347-349.
  3. Neuberger MS, Williams GT, Mitchell EB, Jouhal SS, Flanagan JG, Rabbitts TH. A hapten-specific chimaeric IgE antibody with human physiological effector function. Nature. 1985 Mar 21-27;314(6008):268-70. PubMed PMID: 2580239.
  4. Neuberger, M.S., Williams, G.T. (1986) Construction of novel antibodies by use of DNA transfection: design of plasmid vectors. Philos. Trans. R. Soc. Lond. Ser. A: Hath. Phys. Sci. 317: 425-432.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
0,2 Tris/HCl, pH 7.4 containing 0.09% Sodium Azide as preservative and 5-10% foetal calf serum
Liquid Tissue Culture Supernatant.
The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimaeric human IgM antibody specific for NP has been produced.

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