SM2185P Chimaeric human IgM anti Nitrophenyl-hapten (NP) antibody

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Human anti Chimaeric human IgM anti Nitrophenyl-hapten (NP) THM


Product Description for Chimaeric human IgM anti Nitrophenyl-hapten (NP)

Human anti Chimaeric human IgM anti Nitrophenyl-hapten (NP) THM.
Presentation: Purified
Product is tested for Enzyme Immunoassay.

Properties for Chimaeric human IgM anti Nitrophenyl-hapten (NP)

Product Category Primary Antibodies
Quantity 0.5 mg
Presentation Purified
Applications E
Clonality Monoclonal
Clone THM
Host Human
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Hapten, 4-hydroxy-3-nitrophenylacetyl (NP). Plasmids containing chimaeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line.
Application ELISA.
Concentration 1.0 mg/ml
General Readings
  1. Neuberger MS, Williams GT, Fox RO. Recombinant antibodies possessing novel effector functions. Nature. 1984 Dec 13-19;312(5995):604-8. PubMed PMID: 6095112.
  2. Neuberger, M.S. (1985) Making Novel Antibodies by expressing transfected immunoglobulin genes. TIBS 347-349.
  3. Neuberger MS, Williams GT, Mitchell EB, Jouhal SS, Flanagan JG, Rabbitts TH. A hapten-specific chimaeric IgE antibody with human physiological effector function. Nature. 1985 Mar 21-27;314(6008):268-70. PubMed PMID: 2580239.
  4. Neuberger, M.S., Williams, G.T. (1986) Construction of novel antibodies by use of DNA transfection: design of plasmid vectors. Philos. Trans. R. Soc. Lond. Ser. A: Hath. Phys. Sci. 317: 425-432.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity chromatography on NIP-Cap-Sepharose
Buffer System:
PBS, pH7.2 containing 0.09% Sodium Azide
Liquid purified IgG
The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimaeric human IgM antibody specific for NP has been produced.

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