AM06003PU-N CHK2 antibody

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50 µg / €380.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CHK2 73C175.1.1

Product Description for CHK2

Mouse anti Human CHK2 73C175.1.1.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for CHK2

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Presentation Purified
Reactivity Hu
Applications P, WB
Clonality Monoclonal
Clone 73C175.1.1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O96017
Immunogen:
A synthetic peptide obtained from Human Chk2 protein sequence
GeneID:
11200
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Western Blot: 2 µg/ml.
Background Chk2 (checkpoint kinase 2) is the mammalian homolog of the Saccharomyces cerevisiae Rad53 and is required for the DNA damage and replication checkpoints (1). In response to low-dose ionizing radiation (IR), which occurs in an ataxia telangiectasia mutated (ATM)-dependent manner, Chk2 can phosphorylate the mitosis-inducing phosphatase Cdc25C on an inhibitory site, blocking entry into mitosis, and p53 on a regulatory site, causing G1 arrest (2,3). In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or at low level in static tissues such as muscle or brain (4). Mutations in Chk2 inactivates DNA damage checkpoint pathway involving Chk2 in lung cancer suggesting that reduced expression of Chk2 may be responsible for the development of lung cancer (5). The Chk2-/- knockout cells are resistant to DNA damage-induced apoptosis, defective for p53 stabilization and for induction of p53-dependent transcripts such as p21 in response to gamma irradiation (6).
General Readings
  1. Lukas C, Bartkova J, Latella L, Falck J, Mailand N, Schroeder T, et al. DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology. Cancer Res. 2001 Jul 1;61(13):4990-3. PubMed PMID: 11431331.
  2. Shieh SY, Ahn J, Tamai K, Taya Y, Prives C. Genes Dev 14(3): 289-300 (2000).
  3. Falck J, Mailand N, Syljuasen RG, Bartek J, Lukas J. Nature 410 (6830): 842-847 (2001).
  4. Matsuoka S, Huang M, Elledge SJ. Science 4; 282(5395): 1893-1897 (1998).
  5. Hirao A, Kong YY, Matsuoka S, Wakeham A, Ruland J, Yoshida H, et al. DNA damage-induced activation of p53 by the checkpoint kinase Chk2. Science. 2000 Mar 10;287(5459):1824-7. PubMed PMID: 10710310.
  6. Matsuoka S, Nakagawa T, Masuda A, Haruki N, Elledge SJ, Takahashi T. Reduced expression and impaired kinase activity of a Chk2 mutant identified in human lung cancer. Cancer Res. 2001 Jul 15;61(14):5362-5. PubMed PMID: 11454675.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
PBS containing 0.02% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This antibody detects Serine/Threonine Protein Kinase CHK2 (CHEK2) (Family: Protein Kinase, Subfamily: CDS1).
Species:
Human.
Gene ID 11200
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for CHK2 (6 products)

Catalog No. Species Pres. Purity   Source  

CHK2 (transcript variant 1)

CHK2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

CHK2

CHK2 Human
  Abnova Taiwan Corp.

Positive controls for CHK2 (4 products)

Catalog No. Species Pres. Purity   Source  

CHEK2 293T Cell Transient Overexpression Lysate(Denatured)

CHEK2 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CHEK2 overexpression lysate

CHEK2 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CHEK2 overexpression lysate

CHEK2 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

Chk2 Lysate

Western Blot: Chk2 Lysate [NBL1-09147] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for CHEK2.
  Novus Biologicals Inc.
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